Caffeic acid (CA), 3,4-dihydrocinnamic acid (HCA), 3,4-dimethoxycinnamic acid (DMCA), 1-chloro-2,4-dinitrobenzene (CDNB), reduced glutathione (GSH), oxidized glutathione (GSSG), gly–gly, l -γ-glutamyl-p-nitronilide, NADPH, ATP, cysteine, glutamine, serine, 2,3 naphthalene dicarboxaldehyde (NDA), 5-sulfosalicylic acid (SSA), 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), enzymes and other common reagents were purchased from Sigma Chemical Co., St. Louis, MO. Primary and secondary antibodies were purchased from Abcam, Cambridge, MA. RNA isolation kits, cDNA kits and qRT-PCR reagents were purchased from Agentek, Israel.
3 4 5 dimethylthiazole 2 yl 2 5 diphenyltetrazolium bromide
Manufactured by Merck Group
Sourced in United States
3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide is a chemical compound commonly used as a reagent in cell-based assays. It is a yellow, water-soluble tetrazolium salt that can be reduced to a purple formazan product by metabolically active cells. The formation of the formazan dye is an indicator of cellular activity and can be used to quantify the viability, proliferation, or cytotoxicity of cells in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Serine, by Merck Group (1 mentions)
Primary and secondary antibodies, by Abcam (1 mentions)
Glutamine, by Merck Group (1 mentions)
1 chloro 2 4 dinitrobenzene, by Merck Group (1 mentions)
5 sulfosalicylic acid, by Merck Group (1 mentions)
Oxidized glutathione, by Merck Group (1 mentions)
3 4 dimethoxycinnamic acid, by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Cysteine, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Nadph, by Merck Group (1 mentions)
Reduced glutathione, by Merck Group (1 mentions)
Dicyclohexylcarbodiimide, by Merck Group (1 mentions)
Glyceryl monostearate, by Merck Group (1 mentions)
Carbopol 940, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Sodium hydroxide (naoh), by Beijing Chemical Works (1 mentions)
Hydrochloric acid (hcl), by Beijing Chemical Works (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
7 protocols using 3 4 5 dimethylthiazole 2 yl 2 5 diphenyltetrazolium bromide
1
Glutathione Biosynthesis and Assays
2
Cyclic RGD Peptide-PEG Nanoparticles
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Cyclo (Arg-Gly-Asp-d-Phe-Lys) (cRGDfK) and polyethylene glycol (PEG) were purchased from Xi’an ruixi Biological Technology Co, Ltd (Xi’an, China). D-α-tocopheryl succinate dichloromethane (TOSD) was obtained from Shanghai ZZBIO Co., Ltd (Shanghai, China). Dicyclohexylcarbodiimide (DCC), glyceryl monostearate (GMS), Carbopol 940 (CBP), 3-(4,5-dimethylthiazole-2-yl)−2,5-diphenyltetrazolium bromide (MTT), and Dulbecco’s modified Eagle’s medium (DMEM) were obtained from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was provided by Lonza Bioscience (Walkersville, MD).
3
Antioxidant Evaluation of Valtrate Compounds
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HPLC-grade methanol and acetonitrile were purchased from Fisher Scientific (Fair Lawn, NJ, USA). A Milli-Q water purification system (Millipore, San Diego, CA, USA) was used to further purify distilled water. Other materials included cisplatin (CP), N, N- Dimethylformamide (DMF), 2,2’-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS), 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethylsulfoxide (DMSO), butylated hydroxytoluene (BHT), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) and 2,7-dichlorofluorescein (DCF), all of which were purchased from Sigma Aldrich Co. (St. Louis, MO, USA). Annexin V-fluorescein isothiocyanate (Annexin V-FITC) and a propidium iodide (PI) kit were obtained from BD (San Jose, CA, USA). Valtrate (lot: 111840–201402), acevaltrate (lot: 111841–201401) and 1-β acevaltrate were obtained from the National Institutes for Food and Drug Control of China. The analytical reagent-grade sodium hydroxide, hydrochloric acid and hydrogen peroxide (H2O2, 30%) used in this study were purchased from Beijing Chemical Works (Beijing, China).
4
Molecular Signaling in MCF7 Breast Cancer
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MCF7 (ATCC® HTB-22™) were obtained from the ATCC. Horseradish-peroxidase-conjugated anti-rabbit IgG and anti-mouse IgG antibodies, Alexa488 conjugated anti-rabbit IgG, bacterial collagenase, 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), DCFDA, tetrahydro-2-furoic acid (THFA), glycyl-L-proline, ibuprofen, indomethacin, diclofenac, and sulindac were purchased from Sigma-Aldrich. The celecoxib and troglitazone were products of Targetmol. Solution 5 (VB-48™ PI–AO) was purchased from ChemoMetec. The primary antibodies against: COX2; p53; p-AMPKα; m-TOR; caspase-8; caspase-9, total and cleaved; caspase-7, total and cleaved; PARP, total and cleaved; and B-actin were products of Cell Signaling Technology. The PRODH/POX primary antibodies and PPARγ were products of Santa Cruz Biotechnology. The PYCR1, PYCRL, and PPAR delta primary antibodies were obtained from Abnova. The Hoechst 33342 was obtained from Becton Dickinson.
5
Evaluating Anti-Cancer Drug Sensitivity
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RPMI-1640, DMEM, 3-(4, 5, - dimethylthiazole-2-yl)-2, 5 diphenyltetrazolium bromide (MTT), phenylmethanesulfonyl fluoride (PMSF), cis-diammineplatinum (II) dichloride, EGF, cholera toxin, hydrocortisone, insulin were purchased from Sigma-(Aldrich, MS, USA. DMEM/F12, antibiotic-antimycotic (100×), horse serum, Lipofectamine 2000 was obtained from GIBCO Invitrogen Corporation USA. Antibodies of Akt1, Akt2, Akt3, Vimentin were purchased from Cell signaling technology, Massachusetts, USA. Antibodies for caspase-3, PARP-1 and β -Actin were from Santa Cruz Biotechnology, Texas, USA. Electrophoresis reagents, reagents for protein estimation were from Bio-Rad Laboratories, California, USA. Polyvinyldifluoride (PVDF) membrane was purchased from Millipore, Massachusetts, USA. Aldefluor assay buffer (#01701) and DEAB (#01705) were from STEM-CELL Technologies (Vancouver, BC, Canada). Zosuquidar (LY335979) was obtained through Kanisa Pharmaceuticals Inc (Irvine, CA, USA). Transfection reagent, medium and ABCG2 siRNA (sc41151) were purchased from Santa Cruz Biotechnology.
6
Antifungal Susceptibility Assay Protocol
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All solvents, inorganic salts, Tween 80 and dimethylsulphoxide (DMSO) were purchased from Ciccarelli (San Lorenzo, Argentina). Materials for the culture media were purchased from Britania (Buenos Aires, Argentina). Roswell Park Memorial Institute (RPMI-1640), 3-(N-morpholino) propanesulfonic acid (MOPS), amphotericin B (AMB), fluconazole (FCZ), itraconazole (ITZ), 3-[4,5-dimethylthiazole-2-yl]-2,5diphenyltetrazolium bromide (MTT), nitro blue tetrazolium (NBT), 1,3diphenylisobenzofuran (1,3-DPBF), sterile fetal bovine serum and Bovine Serum Albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 4-aminophenazone, hydroxybenzoate and horseradish peroxidase were included in the enzymatic Glycemia Kit (Wiener Lab, Rosario, Argentina). Aluminum-blacked silica gel 60 F254 TLC layers were purchased from Merck (Darmstadt, Germany).
7
Ceragenin-Attached Gold Nanosystems
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Reagents required for ceragenin-attached gold nanosystems [Citation43] were purchased from Sigma-Aldrich (MO, USA). Laboratory strains of OM-causing pathogens: H. influenzae ATCC 49766, M. catarrhalis ATCC 25238, Streptococcus pneumoniae ATCC 49619 were from American Type Culture Collection (VA, USA). Growth media were as follows: chocolate II Agar, blood No 2 LAB-AGAR+ 5% KB, brain heart infusion broth medium and required supplements: β-nicotinamide adenine dinucleotide (NAD) and hematin porcine were obtained from Becton Dickson (NJ, USA), BioMaxima (Lublin, Poland) and Sigma-Aldrich, respectively. Brain heart infusion broth medium was purchased from Sigma-Aldrich. Reagents for colorimetric and fluorimetric analysis: 1-N-phenylnaphtylamine (NPN), 2′,7′-dichlorofluorescin diacetate (DCFH-DA), Bradford reagent and 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were from Sigma-Aldrich. IL-8 Human ELISA Kit was purchased from Thermo Fisher Scientific (MA, USA).
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