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Genechip mouse st arrays 2

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The GeneChip Mouse ST Arrays 2.0 is a gene expression microarray platform designed for profiling the transcriptome of mouse samples. It provides comprehensive coverage of the mouse genome, allowing for the analysis of over 35,000 well-annotated mouse genes.

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Lab products found in correlation

Genechip wt pico reagent kit, by Thermo Fisher Scientific (2 mentions) Bioanalyzer with nano total rna chips, by Agilent Technologies (2 mentions) Rneasy micro kit, by Qiagen (2 mentions)

2 protocols using genechip mouse st arrays 2

1

Transcriptomic Profiling of Microglia Subsets

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from the eKC and mKC sorted from Cx3cr1-CreERT2R26-tdT mice with the RNeasy Micro Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocol. Quality and quantity of RNA was estimated with Bioanalyzer with Nano total RNA Chips (Agilent, Santa Clara, CA, USA), the RIN values exceeded 7.8 for all samples used for microarray measurement. Total RNA was amplified, fragmented and labeled with biotin using the GeneChip WT Pico Reagent Kit (Affymetrix, Santa Clara, CA, USA) and samples were hybridized to GeneChip Mouse ST Arrays 2.0 according to the manufacturer’s instructions. Probeset signal intensities were background-corrected and normalized using the RMA algorithm. Significantly regulated genes were identified using paired-T tests (pairing by animal, limma package of R/Bioconductor) with Benjamini-Hochberg correction for multiple testing. Genes regulated at least 1.5 fold with p < 0.1 were considered significant. The microarray analysis was done with CARMAweb 1.5 software38 (link).
Theurl I., Hilgendorf I., Nairz M., Tymoszuk P., Haschka D., Asshoff M., He S., Gerhardt L.M., Holderried T.A., Seifert M., Sopper S., Fenn A.M., Anzai A., Rattik S., McAlpine C., Theurl M., Wieghofer P., Iwamoto Y., Weber G.F., Harder N.K., Chousterman B.G., Arvedson T.L., McKee M., Wang F., Lutz O.M., Rezoagli E., Babitt J.L., Berra L., Prinz M., Nahrendorf M., Weiss G., Weissleder R., Lin H.Y, & Swirski F.K. (2016). On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver. Nature medicine, 22(8), 945-951.
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2

Transcriptomic Profiling of Microglia Subsets

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from the eKC and mKC sorted from Cx3cr1-CreERT2R26-tdT mice with the RNeasy Micro Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocol. Quality and quantity of RNA was estimated with Bioanalyzer with Nano total RNA Chips (Agilent, Santa Clara, CA, USA), the RIN values exceeded 7.8 for all samples used for microarray measurement. Total RNA was amplified, fragmented and labeled with biotin using the GeneChip WT Pico Reagent Kit (Affymetrix, Santa Clara, CA, USA) and samples were hybridized to GeneChip Mouse ST Arrays 2.0 according to the manufacturer’s instructions. Probeset signal intensities were background-corrected and normalized using the RMA algorithm. Significantly regulated genes were identified using paired-T tests (pairing by animal, limma package of R/Bioconductor) with Benjamini-Hochberg correction for multiple testing. Genes regulated at least 1.5 fold with p < 0.1 were considered significant. The microarray analysis was done with CARMAweb 1.5 software38 (link).
Theurl I., Hilgendorf I., Nairz M., Tymoszuk P., Haschka D., Asshoff M., He S., Gerhardt L.M., Holderried T.A., Seifert M., Sopper S., Fenn A.M., Anzai A., Rattik S., McAlpine C., Theurl M., Wieghofer P., Iwamoto Y., Weber G.F., Harder N.K., Chousterman B.G., Arvedson T.L., McKee M., Wang F., Lutz O.M., Rezoagli E., Babitt J.L., Berra L., Prinz M., Nahrendorf M., Weiss G., Weissleder R., Lin H.Y, & Swirski F.K. (2016). On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver. Nature medicine, 22(8), 945-951.
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