Autograph ags x 500n

The three-point bending tests were conducted on the femurs to estimate the mechanical properties of the cortical bone.²² (link) Thirty femur bones (five groups, six rats per group) were used for this experiment. Each bone was placed on two lower supports (15-mm span) with the anterior aspect facing down and loaded from above at the midpoint between the two supports. The supporting and loading contacts were round with a diameter of 3 mm. An Autograph AGS-X 500 N model (Shimadzu, Japan) test machine was used with a stroke rate of 5 mm/min. Displacement was measured via a linear variable differential transformer mounted to the crosshead. Load deflection data were recorded at 10 Hz using Trapezium X software and analyzed using an attached personal computer. The same basic extrinsic and intrinsic properties were determined as previously indicated except that the analysis for estimating intrinsic properties invoked classical beam theory assumptions in this case.²² (link)

Biomechanical properties of femoral bone were evaluated using three-point bending test method (Shimadzu, Autograph AGS-X 500 N, Japan) controlled by proprietary software (Trapezium Version 1.4.2, Shimadzu). The femurs were cleaned from soft tissues and the total length and width of the midshaft were measured with digital caliper. The left femur from each rat was placed on an adjustable two-point block jig spaced 5 mm apart, with a load sensor attached to a bending punch on the crosshead. Force was applied at a rate of 10 mm/minute to the mid-shaft of the femur diaphysis until a break was determined by measuring a reduction in force. The recorded testing parameter included both extrinsic and intrinsic properties. Whole-bone mechanical properties were determined by the extrinsic parameters (force and displacement), while the measurement of the bone material were determined by intrinsic parameters, stress and strain.