Tdf 100a kit

Acetonitrile 99.9%, n-hexane 95% and ethyl acetate 99.8% were of HPLC grade from Fisher Scientific (Lisbon, Portugal) . The fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St. Louis, MO, USA), as also other individual fatty acid isomers and standards of sugars (D-(+)mannitol, D-(+)-trehalose), tocopherols (α-, β-, and γ-isoforms), organic acids (oxalic and fumaric acids), phenolic compounds (gallic, p-hydroxybenzoic, protocatechuic, cinnamic and p-coumaric acids), trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2carboxylic acid) , and dietary fiber enzyme kit (TDF-100A Kit), pepsin enzyme (P-7000), pancreatin enzyme (P-1750 ) and porcine bile (B-8631). Racemic tocol, 50 mg/mL, was purchased from Matreya (PA, USA). Micro (Fe, Cu, Mn and Zn) and macroelements (Ca, Mg, Na and K) standards (> 99% purity), as well LaCl 2 and CsCl (> 99% purity) were purchased from Merck (Darmstadt, Germany). Anthrone was obtained from Panreac Química (Barcelona, Spain). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).