The morphology of phage particles was studied using transmission electron micros-copy (TEM). Concentrated purified samples of phage Murka were placed on grids and stained with 1% aqueous uranyl acetate (pH 4.0). Prepared grids were examined using a JEM-2100 200 kV transmission electron microscope (JEOL, Tokyo, Japan). The dimensions of each phage were averaged among ~20 individually measured particles.
Jem 2100 200 kv transmission electron microscope
Manufactured by JEOL
Sourced in Japan
The JEM-2100 is a 200 kV transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of samples at the nanoscale. The JEM-2100 features a LaB6 electron source and can achieve a resolution of up to 0.23 nm.
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5 protocols using jem 2100 200 kv transmission electron microscope
1
Phage Particle Morphology by TEM
2
Purification and Visualization of Phages
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To obtain preparations for microscopy, 100 mL of host culture was grown and prophage was induced, as described above. The resulting lysate was then concentrated and purified, according to the protocol described by Ackermann [123 ]. Centrifugation with ammonium acetate was carried out twice. Concentrated purified samples were placed on grids and stained with 1% aqueous uranyl acetate (pH 4.0). Prepared grids were examined using a JEM-2100 200 kV transmission electron microscope (JEOL, Tokyo, Japan).
3
Morphological Analysis of Psg Phages
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Negative-staining transmission electron microscopy [57 ] was performed to assess the morphology of isolated Psg phages. Aliquots of the purified phages were loaded onto a carbon-coated copper grid, subjected to glow-discharge and then negatively stained with 1% uranyl acetate for 30 s and air-dried. Prepared grids were examined using a JEM-2100 200 kV transmission electron microscope (JEOL, Tokyo, Japan). The dimensions of each phage were averaged among ~20 individually measured particles.
4
Bacteriophage Visualization by TEM
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Bacteriophage was visualized by a transmission electron microscope (TEM) as described by Kim et al. (2019) (link) with some modifications. A carbon-coated copper grid was placed onto a drop of purified bacteriophage (1010 PFU/mL) for 2 min. The grid with the sample was negatively stained first with 2% (w/v) uranyl acetate for 2 min, followed by sterile water for 1 min. The excess sample was removed with a filter paper, and the grid was allowed to air dry for 10 min. The morphology of the bacteriophage was then visualized with a JEM-2100 200-kV transmission electron microscope (JEOL, Japan).
5
Morphology of Phage Ayka by TEM
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The morphology of phage particles was studied using transmission electron microscopy (TEM). Concentrated purified samples of phage Ayka were placed on grids and stained with 1% aqueous uranyl acetate (pH 4.0). Prepared grids were examined using a JEM-2100 200 kV transmission electron microscope (JEOL, Tokyo, Japan). The dimensions of each phage were averaged among ~20 individually measured particles.
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