Anti src and anti phospho src antibodies

Anti-Bcl-2, anti-Bax, anti-caspase-3, anti-focal adhesion kinase-1 (FAK-1), anti-ERK1/2, anti-phospho-ERK1/2, anti-β-actin, anti-mouse IgG-FITC and polymerised HRP-anti Rb IgG antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-Akt and anti-phospho-Akt antibodies were obtained from Signalway Antibody (College Park, MD, USA). Anti-CXCR4 was purchased from Abcam (Cambridge, MA, USA). Anti-Src and anti-phospho-Src antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Hoechst 33258 was purchased from Sigma-Aldrich (St Louis, MO, USA). In Situ Cell Apoptosis Detection Kit II was purchased from Wuhan Boshide Biotechnology (Wuhan, China). ElivisionTM plus Polyer HRP (mouse/rabbit) IHC Kit was purchased from Maixin Biology (Fuzhou, China). TdT-mediated dUTP nick end labelling (TUNEL) kit was obtained from Wuhan Boster Biological Technology (Beijing, China). Human-SDF-1α was purchased from PeproTech Systems (Rocky Hill, NJ, USA). AMD3100, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and fibronectin were obtained from Sigma-Aldrich.

The following mouse monoclonal antibodies were used: anti-FAK (BD Transduction Laboratories, Le Pont de Claix, France), anti-cortactin (Millipore, Molsheim, France), anti-Tks-5 (Tebu-bio, Le Perray-en-Yvelines, France), anti-Tks-5 PX domain, anti-Tks-5 SH3 domain, anti-phosphotyrosine (Millipore), anti-talin, anti-paxillin, anti-phospho-Y397 FAK (Invitrogen, Camarillo, CA, USA) and anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA). Anti-Src and anti-phospho-Src anti-bodies were from Cell Signalling (Danvers, MA, USA) or Invitrogen (Grand Island, NY, USA). Horseradish peroxidase-conjugated goat anti-mouse was from Promega (Madison, WI, USA). Actin antibody conjugated to Alexa fluor 647 was from Santa Cruz (Heidelberg, Germany). PP2 was from Calbiochem (Billerica, MA, USA) and MnTMPyP from MERCK Biosciences (Darmstadt, Germany). G-sepharose beads and ECL^plus kit were from GE healthcare (Freiburg, Germany). MnPyP, CM-H2DCF-DA, Alexa Fluor 488-conjugated goat anti-mouse, Alexa-405-labelling kit and Lipofectamine 2000 were from Invitrogen. Gelatin from porcine skin and mouse monoclonal Ab was from Sigma-Aldrich.