The bispecific antibody sequence contained vectors were transfected into CHO-K1 cells and then antibiotic selection was applied for screening stable cell pools. For the BsAbs production, cells were cultivated in Dynamis™ medium for 14 days and harvested by centrifugation. Monoclonal antibodies mAb1 and mAb2 were transiently expressed by CHO-K1 cells and cultivated in CD-CHO production medium for 10 days. For purification, all the proteins were bound and eluted from MabSelect SuRe protein A column (Cytiva, MA, United States), then buffer exchanged into 10 mM sodium acetate solution.
Mabselect sure protein a column
Manufactured by Cytiva
Sourced in United States
The MabSelect SuRe protein A column is a chromatography resin designed for the purification of monoclonal antibodies (mAbs) and Fc-containing proteins. The resin features protein A ligands that are engineered for enhanced stability and alkaline resistance, enabling the use of harsh cleaning conditions. The column provides high dynamic binding capacity and recovers purified proteins with high purity and yield.
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Lab products found in correlation
2 protocols using mabselect sure protein a column
1
Bispecific Antibody Production in CHO Cells
2
CAR T Cell Activation Assay
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Anti-huk666 idiotype and anti-fmc63 idiotype were generated in house from expi-CHO cells (Thermo Fisher Scientific, A29127) and purified using mAb Select SuRe protein A column (Cytiva, 11003494) couple with Akta Pure system (see Supplementary Fig. S1 for flow cytometry characterization).
Ten micrograms of anti-huk666 idiotype (for GD2-specific CAR T Cells) or 1 μg of anti-fmc63 idiotype (for CD19-specific CAR T cells) was coated on non-tissue culture–treated 24-well plates for 16 hours at 4°C. Plates were washed twice before plating T cells (1 × 106 cells/well). Plates were centrifuged at 500 × g for 5 minutes and incubated at 37°C for 24 to 48 hours.
Ten micrograms of anti-huk666 idiotype (for GD2-specific CAR T Cells) or 1 μg of anti-fmc63 idiotype (for CD19-specific CAR T cells) was coated on non-tissue culture–treated 24-well plates for 16 hours at 4°C. Plates were washed twice before plating T cells (1 × 106 cells/well). Plates were centrifuged at 500 × g for 5 minutes and incubated at 37°C for 24 to 48 hours.
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