Ampicillin

Manufactured by Teknova

Sourced in United States

Ampicillin is a broad-spectrum antibiotic used in laboratory settings. It is effective against a variety of gram-positive and gram-negative bacteria. Ampicillin functions by inhibiting the synthesis of the bacterial cell wall, leading to cell death.

Automatically generated - may contain errors

Lab products found in correlation

Kanamycin sulfate, by IBI Scientific (1 mentions) Neomycin sulfate, by Fagron (1 mentions) Balb cj mice, by Jackson ImmunoResearch (1 mentions) Balb cj wild type mice, by Jackson ImmunoResearch (1 mentions) Metronidazole, by Spectrum Chemical (1 mentions) X gal, by Teknova (1 mentions) Isopropyl β d 1 thiogalactopyranoside (iptg), by Teknova (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Lps from e coli o111 b4, by Merck Group (1 mentions) Vancomycin, by Merck Group (1 mentions) Metronidazole, by Merck Group (1 mentions) Kanamycin, by Teknova (1 mentions) Ptwist, by Twist Bioscience (1 mentions) Zymopure 2 plasmid maxiprep kit, by Zymo Research (1 mentions)

4 protocols using ampicillin

Antibiotic-Mediated Gut Microbiome Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

All animal experiments were performed in accordance with relevant guidelines and regulations. The study protocol was approved by Institutional Animal Care and Use Committee (IACUC) of the University at Buffalo and all methods were carried out in compliance with the ARRIVE guidelines. Wild-type BALB/cJ mice (Jackson Laboratory; Cat#000651) were given 1 mg/ml of Kanamycin sulfate (IBI Scientific; Cat# 25389–94-0) via autoclaved drinking water for 5 days to suppress host microbiota. After 2 days of washout period, approximately 1 × 10⁹ CFU of P. gingivalis was administered via oral gavage using stainless steel feeding tube (see below for P. gingivalis cultivation method). Interleukin 10 (Il10)-deficient BALB/cJ mice (Jackson Laboratory; Cat#004333) were given antibiotic cocktail of 1 mg/ml each of ampicillin (Teknova; Cat#50–841-073), neomycin sulfate (Fagron; Cat#804599), metronidazole (Spectrum Chemical; Cat#M1284), and 0.5 mg/ml of Vancomycin hydrochloride (Fagron; Cat#804147) via drinking water for 4 weeks (refilled every 96 h) to ablate host microbiota (See below for cecal microbiota transplantation method). All animals were single housed and raised in sealed positive pressure cages (Allentown).

Sohn J., Li L., Zhang L., Settem R.P., Honma K., Sharma A., Falkner K.L., Novak J.M., Sun Y, & Kirkwood K.L. (2022). Porphyromonas gingivalis indirectly elicits intestinal inflammation by altering the gut microbiota and disrupting epithelial barrier function through IL9-producing CD4+ T cells. Molecular oral microbiology, 37(2), 42-52.

+ Open protocol

+ Expand

Evaluating Transformation Efficiencies of Alkali-Heat Treated Plasmid DNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transformation efficiencies for alkali and heat treated DNA were evaluated using competent E. coli DH5 alpha (Invitrogen-Thermo Fisher Scientific), JM109 and BL21(DE3) pLys S (Promega), and 60 ng alkali and heat treated pBlueScript II plasmid (Agilent Technologies) or untreated plasmid as control. Bacterial transformations were performed using a variation of the heat shock method described previously [18 ], prior to plating on LB agar containing 100 μg ml⁻¹ ampicillin, 80 μg ml⁻¹ X-gal, and 50 mM IPTG (Teknova). Expression and function of the LacZ gene product from pBlueScript II was evaluated by blue white screening of DH5 alpha colonies, and transformation efficiencies were calculated in colony forming units per μg of plasmid.

Lemire K.A., Rodriguez Y.Y, & McIntosh M.T. (2016). Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA. Virology Journal, 13, 88.

+ Open protocol

+ Expand

Antibiotic and LPS Treatment in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

A variation of a published protocol (Rakoff-Nahoum et al., 2004 (link)) was used. Six-seven week old female mice were given drinking water containing 1g/l of ampicillin (Teknova), 1g/l of streptomycin (Gibco), 1 g/l of metronidazole (Sigma) and 1 g/l of vancomycin (Sigma) for 1–4 weeks. For LPS supplementation mice were given 50 µg/ml of LPS from E.coli O111:B4 (Sigma) in drinking water (Rakoff-Nahoum et al., 2004 (link)) together with antibiotics for 4 weeks.

Muller P.A., Koscsó B., Rajani G.M., Stevanovic K., Berres M.L., Hashimoto D., Mortha A., Leboeuf M., Li X.M., Mucida D., Stanley E.R., Dahan S., Margolis K.G., Gershon M.D., Merad M, & Bogunovic M. (2014). Crosstalk between Muscularis Macrophages and Enteric Neurons Regulates Gastrointestinal Motility. Cell, 158(2), 300-313.

+ Open protocol

+ Expand

Antibody Plasmid Construction and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The R175H antibody plasmids encoding the heavy-chain and light-chain¹⁵ (link) were synthesized, and the antibody was purified by Syd Labs, Inc. The single-chain variable fragment (scFv) was obtained from a previous study.¹⁷ (link) Assembly of antibody heavy- and light-chain DNAs into a mammalian expression vector pTwist (Twist Bioscience, South San Francisco, CA, USA) or gWIZ (Aldevron, Fargo, ND, USA) was performed to construct pR175H-mAb or pR175H/mCD3-BsAb. The plasmids were transformed into competent Escherichia coli cells and propagated in Luria–Bertani (LB) broth supplemented with 100 μg/mL ampicillin (Teknova, Inc., Hollister, CA, USA) or 50 μg/mL kanamycin (Teknova, Inc.). Plasmids were purified from DH5α cells grown overnight using an endotoxin-free ZymoPURE™ II Plasmid Maxiprep Kit (Zymo Research, Irvine, CA, USA).

Chai D., Wang X., Neeli P., Zhou S., Yu X., Sabapathy K, & Li Y. (2023). DNA-delivered monoclonal antibodies targeting the p53 R175H mutant epitope inhibit tumor development in mice. Genes & Diseases, 11(4), 100994.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!