99mtc maa

HMPAO (Ceretec®) was purchased in kit form from GE Healthcare (Arlington Heights, IL), and technetium-labeled macroaggregated albumin (^99mTc-MAA, particle sizes 20 – 40 μm) was purchased from Cardinal Health (Wauwatosa, WI). Antibodies to 3-nitrotyrosine (3-NT) (Abcam, # ab52309), and keratin 4-hydroxynonenal (4-HNE) (Abcam, # ab46545) were used with appropriate secondary antibodies (mouse 1:3000 for 3-NT and rabbit 1:3000) to measure expression of 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) in lung tissue homogenate. Diethyl maleate (DEM) and other reagent grade chemicals were purchased from Sigma-Aldrich (St. Louis, MO).

Duramycin (3,035 g/mole MW) kits were prepared as previously described [12 (link)], and technetium-labeled macroaggregated albumin (^99mTc-MAA, particle sizes 20 – 40 μm) was purchased from Cardinal Health (Wauwatosa, WI). Antibodies to cleaved caspase 3 (Biocare, #CP229B), CD31 (Acris, #AP15436PU-N), keratin 7 (Abcam, #9021), and myeloperoxidase (Abcam, #45977) were used with appropriate secondary antibodies (Jackson Immuno #711-066-152 for CD31, Jackson Immuno #715-066-151 for keratin 7, and Abcam #6829 HRP for MPO) to identify endpoints of interest. Other reagent grade chemicals were purchased from Sigma Chemical Company.