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Mini vortex mixer

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The Mini Vortex Mixer is a compact, versatile laboratory device designed to gently mix a variety of samples. It provides a consistent vortexing motion to thoroughly mix small volumes of liquids, powders, or suspensions in test tubes, microcentrifuge tubes, or other containers.

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Lab products found in correlation

Ppg1000, by Merck Group (3 mentions) Pox5000, by Merck Group (3 mentions) Methocel e3lv, by Dow (3 mentions) Pluronic f68 f68, by BASF (3 mentions) Genbox anaer, by bioMérieux (1 mentions) 0.45 μm syringe filter, by Thermo Fisher Scientific (1 mentions)

6 protocols using mini vortex mixer

1

Ethanol Extraction of Plant Samples

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One gram of the samples was weighed and extracted in 20 mL of 70% ethanol (1:20 m/v), using ultrasound-assisted extraction for 15 min at 25 °C (PEX05 25 kHz, Reus France). At 7.5 min, the crude extracts were agitated for 30 s using a vortex (VWR mixer mini vortex, EU). The resulting solutions were filtered twice: first under vacuum using glass sintered filters (Redisep 25 g 15–45 µm filters), then using 0.22 µm PTFE filters (Restek, France) into glass vials. Three analytical replicates were prepared for each species and stored at −20 °C until analysis.
The remaining filtrates of 15 mL were dried using a Speedvac (Thermo Scientific Savant Speedvac Concentrator SPD131DDA, equipped with a Thermo Scientific Savant Refrigerated Vapor Trap RVT5105 and an Edwards Pump RV8), then freeze-dried (Cryotec, France). The dried extracts were stored in air-tight containers at ambient temperature in the dark.
Breaud C., Lallemand L., Mares G., Mabrouki F., Bertolotti M., Simmler C., Greff S., Mauduit M., Herbette G., Garayev E., Lavergne C., Cesari M., Bun-Llopet S.S., Baghdikian B, & Garayev E. (2022). LC-MS Based Phytochemical Profiling towards the Identification of Antioxidant Markers in Some Endemic Aloe Species from Mascarene Islands. Antioxidants, 12(1), 50.
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2

Bacterial Enumeration by Dilution Plating

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After ultrasonication, the samples were vortexed for 30 s at level 9 using a Vortex mixer (Mixer mini Vortex, VWR International bvba, Radnor, USA). A dilution series up to 1:104 was prepared with 0.9% NaCl for each sample. The diluted samples were again vortexed before plating onto Columbia blood agar (CBA; Oxoid GmbH, Wesel, Germany) and yeast-cysteine blood agar (HCB-agar, Institute of Medical Microbiology and Hygiene, Freiburg, Germany). One hundred microliters of the diluted solution was dispensed onto the culture medium using a sterile glass spatula. CBA was used for cultivating aerobic bacteria, which were incubated for 3–5 days in 5% CO2 atmosphere and 36 °C. HCB-agar was used for cultivating anaerobic bacteria, which were incubated for 10 days at 36 °C. An anaerobic pot and a gas generator (GENbox anaer, BioMérieux®, Marcy-I Étoile, France) were used to create the proper conditions for anaerobic growth. The number of CFU was determined afterwards for each plate using a colony counter with a magnifying glass (WTW BZG 40, Weilheim, Germany).
Rux C., Wittmer A., Stork A., Vach K., Hellwig E., Cieplik F, & Al-Ahmad A. (2023). Optimizing the use of low-frequency ultrasound for bacterial detachment of in vivo biofilms in dental research—a methodological study. Clinical Oral Investigations, 28(1), 19.
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3

Foam Stability of Polymer Solutions

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Example 12

Solutions of concentration 1000 ppm w/v of poly(propylene glycol) with Mn 1000 (PPG1000, Sigma Aldrich, St. Louis, Mo.), poly(2-ethyl-2-oxazoline) with Mn 5000 and PDI≤1.2 (POX5000, Sigma Aldrich, St. Louis, Mo.), Methocel E3LV (Dow Chemical Company, Midland, Mich.), polysorbate 80 (PS80, Sigma Aldrich, St. Louis, Mo.), and Pluronic F68 (F68, BASF Corporation) were prepared using ultrapure deionized water (18.2 MΩ·cm at 25° C.). 3 mL of each solution was placed into a 5-mL polypropylene tube and vortexed on a Mini Vortex Mixer (VWR, Radnor, Pa.) on mixer setting #8 for 15 seconds. The foam heights were measured after vortexing and are listed in Table 20. The times for the foams to dissipate were also recorded and are listed in Table 20. The foams for the POX5000 and F68 samples did not dissipate over the course of the experiment. The stopping points are listed in Table 20.

TABLE 20
ExcipientFoam height (in)Foam dissipation time (s)
PPG10000.254
POX50000.6327
Methocel E3LV1.00>1125
PS800.257
F681.25>1125

US10016513B2. Stabilizing excipients for therapeutic protein formulations (2018-07-10). REFORM BIOLOGICS, LLC [US]. Inventors: David S. Soane [US], Robert P. Mahoney [US], Philip Wuthrich [US], Daniel G. Greene [US].
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4

Foam Stability of Polymer Solutions

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Example 12

Solutions of concentration 1000 ppm w/v of poly(propylene glycol) with Mn 1000 (PPG1000, Sigma Aldrich, St. Louis, Mo.), poly(2-ethyl-2-oxazoline) with Mn 5000 and PDI≤1.2 (POX5000, Sigma Aldrich, St. Louis, Mo.), Methocel E3LV (Dow Chemical Company, Midland, Mich.), polysorbate 80 (PS80, Sigma Aldrich, St. Louis, Mo.), and Pluronic F68 (F68, BASF Corporation) were prepared using ultrapure deionized water (18.2 MΩ·cm at 25° C.). 3 mL of each solution was placed into a 5-mL polypropylene tube and vortexed on a Mini Vortex Mixer (VWR, Radnor, Pa.) on mixer setting #8 for 15 seconds. The foam heights were measured after vortexing and are listed in Table 20. The times for the foams to dissipate were also recorded and are listed in Table 20. The foams for the POX5000 and F68 samples did not dissipate over the course of the experiment. The stopping points are listed in Table 20.

TABLE 20
ExcipientFoam height (in)Foam dissipation time (s)
PPG10000.254
POX50000.6327
Methocel E3LV1.00>1125
PS800.257
F681.25>1125

US10279048B2. Stabilizing excipients for therapeutic protein formulations (2019-05-07). REFORM BIOLOGICS, LLC [US]. Inventors: David S. Soane [US], Robert P. Mahoney [US], Philip Wuthrich [US], Daniel G. Greene [US].
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5

Foam Characterization of Polymeric Excipients

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Example 12

Solutions of concentration 1000 ppm w/v of poly(propylene glycol) with Mn 1000 (PPG1000, Sigma Aldrich, St. Louis, Mo.), poly(2-ethyl-2-oxazoline) with Mn 5000 and PDI ≤1.2 (POX5000, Sigma Aldrich, St. Louis, Mo.), Methocel E3LV (Dow Chemical Company, Midland, Mich.), polysorbate 80 (PS80, Sigma Aldrich, St. Louis, Mo.), and Pluronic F68 (F68, BASF Corporation) were prepared using ultrapure deionized water (18.2 MΩ·cm at 25° C.). 3 mL of each solution was placed into a 5-mL polypropylene tube and vortexed on a Mini Vortex Mixer (VWR, Radnor, Pa.) on mixer setting #8 for 15 seconds. The foam heights were measured after vortexing and are listed in Table 20. The times for the foams to dissipate were also recorded and are listed in Table 20. The foams for the POX5000 and F68 samples did not dissipate over the course of the experiment. The stopping points are listed in Table 20.

TABLE 20
ExcipientFoam height (in)Foam dissipation time (s)
PPG10000.254
POX50000.6327
Methocel E3LV1.00>1125
PS800.257
F681.25>1125

US10610600B2. Stabilizing excipients for therapeutic protein formulations (2020-04-07). REFORM BIOLOGIES, LLC [US]. Inventors: David S. Soane [US], Robert P. Mahoney [US], Philip Wuthrich [US], Daniel G. Greene [US].
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6

Hydrogel Synthesis from ETTMP and PEGDA

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ETTMP and PEGDA
are purified using a 3.8 cm column of basic aluminum oxide to remove
the radical inhibitor hydroquinone monomethyl ether (MEHQ) from PEGDA
and degraded mercaptopropionic acid from ETTMP, which are further
purified using a 0.45 μm syringe filter (Fisher Scientific,
Waltham, Massachusetts) to remove any remaining alumina particles.
The ETTMP and PEGDA are added to a vial in a 2:3 stoichiometric ratio
and vortexed for 15 s (Mini Vortex Mixer, VWR, Radnor, Pennsylvania).
PBS solution (0.1 M, pH 7.40) is then added to the vial to create
the desired hydrogel formulation. The mixture is then vortexed for
15 s, yielding a clear solution that forms a clear hydrogel. Polymer
mass fractions around 0.15 begin to approach the limit of gel formation.
Rockwell P.N., Maneval J.E., Vogel B.M, & Jablonski E.L. (2023). Water Diffusion and Uptake in Injectable ETTMP/PEGDA Hydrogels. The Journal of Physical Chemistry. B, 127(22), 5055-5061.
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