Dneasy blood tissue kit

Manufactured by Illumina

Sourced in Germany

The DNeasy Blood & Tissue Kit is a laboratory equipment product designed for the extraction and purification of DNA from a variety of sample types, including blood, tissues, and cultured cells. The kit utilizes a silica-membrane-based technology to efficiently capture and purify DNA, making it suitable for a wide range of downstream applications.

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Lab products found in correlation

Truseq nano dna library, by Illumina (1 mentions) Hiseq 2500, by Illumina (1 mentions) Hiseq 2000 sequencing system, by Illumina (1 mentions) Sureselectxt custom target enrichment kit, by Agilent Technologies (1 mentions) Infinium humancytosnp 12, by Illumina (1 mentions)

4 protocols using dneasy blood tissue kit

Identifying Mutational Landscape in Mouse and Human OSCC

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Genomic DNA was prepared from RP-MOC1 cells in culture (Qiagen DNeasy Blood & Tissue Kit, Hilden, Germany) and subjected into Illumina libraries according to the manufacturer’s protocol (Illumina Inc, San Diego, CA, USA). Mutation reads were normalized to the reference C57BL/6NCr genome (mm10 genome) and a genomic database (GENCODE GRCm38.75) of 18 commonly used strains of inbred laboratory mice. Mutational data on human OSCC was obtained from publicly available data in TCGA; cBioPortal (http://www.cbioportal.org/public-portal/). The identified SNPs were subjected to Gene Set Enrichment Analysis (GSEA) pathway analysis [44 (link)].

Vincent-Chong V.K, & Seshadri M. (2020). Development and Radiation Response Assessment in A Novel Syngeneic Mouse Model of Tongue Cancer: 2D Culture, 3D Organoids and Orthotopic Allografts. Cancers, 12(3), 579.

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Genomic DNA Isolation and Illumina Sequencing

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One to two million PnM cells were used to isolate Genomic DNA using Qiagen DNeasy Blood & Tissue Kit, and ~0.5 μg genomic DNA was used for the generation of an Illumina TruSeq Nano DNA Library. The library was 150 base pair paired-end sequenced using Illumina HiSeq2500 at TUCF Genomics core.

AlHaj Abed J., Erceg J., Goloborodko A., Nguyen S.C., McCole R.B., Saylor W., Fudenberg G., Lajoie B.R., Dekker J., Mirny L.A, & Wu C.T. (2019). Highly structured homolog pairing reflects functional organization of the Drosophila genome. Nature Communications, 10, 4485.

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Targeted Panel Sequencing of Melanoma Samples

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DNA from patients and/or PDX were characterized by massively parallel sequencing using a custom-designed 108 gene targeted panel. Results were annotated for mutations, insertions and deletions, and copy number changes. A detailed description of the methodology and analysis is provided in (Garman et al., 2017 ). Briefly, DNA was purified (DNeasy Blood & Tissue Kit), 500 ng of genomic DNA was sheared randomly into 200 bp fragments, and sheared DNA was A-tailed and ligated with adaptor-embedded indexes using the NEBNext® UltraTM DNA Library Prep Kit for Illumina® (New England BioLabs, Inc., Ipswich, MA). Samples were equimolarly pooled prior to capture with a 2.2 Mbp SureSelectXT Custom Target Enrichment Kit (Agilent Technologies, Santa Clara, CA) targeting 108 genes previously implicated in melanomagenesis. Paired-end (2X100 bp) sequencing was performed on the HiSeqTM 2000 sequencing system (Illumina, Inc., San Diego, CA).
To account for mouse DNA contamination, previously unreported variants with an allelic fraction of less than 0.15 were filtered out of the analysis.

Krepler C., Sproesser K., Brafford P., Beqiri M., Garman B., Xiao M., Shannan B., Watters A., Perego M., Zhang G., Vultur A., Yin X., Liu Q., Anastopoulos I.N., Wubbenhorst B., Wilson M.A., Xu W., Karakousis G., Feldman M., Xu X., Amaravadi R., Gangadhar T.C., Elder D.E., Haydu L.E., Wargo J.A., Davies M.A., Lu Y., Mills G.B., Frederick D.T., Barzily-Rokni M., Flaherty K.T., Hoon D.S., Guarino M., Bennett J.J., Ryan R.W., Petrelli N.J., Shields C.L., Terai M., Sato T., Aplin A.E., Roesch A., Darr D., Angus S., Kumar R., Halilovic E., Caponigro G., Jeay S., Wuerthner J., Walter A., Ocker M., Boxer M.B., Schuchter L., Nathanson K.L, & Herlyn M. (2017). A comprehensive patient-derived xenograft collection representing the heterogeneity of melanoma. Cell reports, 21(7), 1953-1967.

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Genomic DNA Extraction and SNP Analysis

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Genomic DNA from the indicated PSC lines was extracted using the QIAGEN DNeasy Blood & Tissue kit and analyzed on the Illumina Infinium HumanCytoSNP-12 (iPSC17.1-2) or CytoSNP850K (iPSC22.1) platforms.

Strano A., Tuck E., Stubbs V.E, & Livesey F.J. (2020). Variable Outcomes in Neural Differentiation of Human PSCs Arise from Intrinsic Differences in Developmental Signaling Pathways. Cell Reports, 31(10), 107732.

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