Adipogenesis differentiation medium
Adipogenesis Differentiation Medium is a cell culture medium designed to support the differentiation of preadipocytes into mature adipocytes. It provides the necessary components to induce and maintain adipogenic differentiation in vitro.
Lab products found in correlation
9 protocols using adipogenesis differentiation medium
Evaluating Mesenchymal Stem Cell Potency
Adipogenesis Induction in Mesenchymal Stem Cells
Differentiation and Doxorubicin Cytotoxicity
Cellular growth media of DMEM (Dulbecco’s modified Eagle’s medium; 1 gram per liter of D-glucose), adipogenesis differentiation medium, and osteogenesis differentiation medium were purchased form Gibco Company (USA) and were then supplemented with penicillin antibiotic (50 units per mL), fungicide amphotericin B (2.5 µg per mL), essential amino acids, and L-glutamine. Doxorubicin drug was purchased from Sigma Company. A solution of 1000 μM in phosphate buffer saline (PBS) was prepared, and the stock solution was kept at -20 oC. Alizarin Red, Oil Red O, and propidium iodide (PI) were obtained from Sigma Company (USA). Fetal calf serum (FCS) and trypsin/EDTA (0.5%) were purchased from Gibco Company (USA), and collagen was supplied from STEM CELL Technologies (Canada). Monoclonal antibodies against surface markers were also provided from Dako Company (Denmark). The measurement kit of cytotoxicity detection for measuring lactate dehydrogenase (LDH) activity was purchased from Roche (Germany), and the solvents were supplied from Merk (Germany).
Quantitative Analysis of Stem Cell Differentiation
For the chondrogenesis assay, pellets were prepared by spinning down 1 × 105 cells and incubating in a 15-mL conical tube in chondrogenesis differentiation medium (Invitrogen) with the medium changed every 3 days. After 28 days of culturing, cells were fixed with 4% formaldehyde, and stained with Alcian Blue (Merck, Darmstadt, Germany).
Directed Differentiation of Mesenchymal Stem Cells
Adipogenic Differentiation Assay Protocol
Bimatoprost Enhances Adipocyte Differentiation
Multilineage Differentiation of Progenitor Cells
StemPro™ osteogenesis differentiation medium, adipogenesis differentiation medium and
chondrogenesis differentiation medium (Thermo Fisher Scientific), respectively. After
14–21 days induction, cells were fixed using 4% paraformaldehyde for 30 min. For
adipogenic differentiation, cells were stained with 2% Oil Red O for 10 min. For
osteogenic differentiation, cells were stained with 1% Alizarin Red S for 3 min. For
chondrogenic differentiation, cells were stained with 1% Alcian Blue solution prepared in
0.1 N HCl for 30 min. The images were visualized under a bright-field microscope.
Multilineage Differentiation of Canine ADSCs
For osteogenic differentiation, passage 2 cADSC were seeded on 12-well plates at a concentration 2 × 105 cells/well and incubated in DMEM supplemented with 10% FBS and 1% antibiotic-antimycotic solution for 24 h. The medium was then replaced with 1 mL of osteogenesis differentiation medium (Thermo Fisher Scientific) and changed every 3 days. For osteogenic analysis, mineral deposits were analyzed quantitatively by Alizarin Red staining after 21 days.
For chondrogenic differentiation, 2 × 105 passage 2 cADSC were suspended in 1 mL of chondrogenic differentiation medium (STEMCELL Technologies, Vancouver, BC, Canada) and 0.5 mL was aliquoted into a 15 mL conical tube and then centrifuged at 300× g for 5 min. The lid was then loosened and the cells were incubated for 3 days, after which 0.5 mL of medium was added and replaced every 3 days. Chondrogenic differentiation was evaluated by Alcian Blue staining after 21 days.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!