Cfu gm

Manufactured by STEMCELL

The CFU-GM assay is a laboratory technique used to quantify the number of granulocyte-macrophage colony-forming units (CFU-GM) present in a sample. CFU-GM are a subset of hematopoietic stem and progenitor cells that can give rise to granulocytes and macrophages. The assay measures the ability of these cells to proliferate and differentiate into colonies of granulocytes and macrophages when cultured in a semi-solid medium.

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Lab products found in correlation

L butionine sulfoxamine bso, by Merck Group (2 mentions) Methocult gf m3534, by STEMCELL (1 mentions) Methocult gf m3534 medium, by STEMCELL (1 mentions)

2 protocols using cfu gm

CFU-GM Assay of Bone Marrow Cells

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Bone marrow cells (2×10⁴) from WT or CGD mice were seeded in semisolid Methocult GF M3534 medium containing rmSCF, rmIL-3 and rhIL-6 for detection of CFU-GM (Stem Cell Technologies). L-butionine-sulfoxamine (BSO, Sigma-Aldrich) was added to methylcellulose media at the indicated concentrations at the time of plating.

Kwak H.J., Liu P., Bajrami B., Xu Y., Park S.Y., Nombela-Arrieta C., Mondal S., Sun Y., Zhu H., Chai L., Silberstein L.E., Cheng T, & Luo H.R. (2015). Myeloid cell-derived reactive oxygen species externally regulate the proliferation of myeloid progenitors in emergency granulopoiesis. Immunity, 42(1), 159-171.

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Quantifying CFU-GM Colonies in Murine Bone Marrow

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Bone marrow cells (2×10⁴) from WT or CGD mice were seeded in semisolid Methocult GF M3534 medium containing rmSCF, rmIL-3 and rhIL-6 for detection of CFU-GM (Stem Cell Technologies). Number of colonies that contained more than 50 cells was counted on day 7. Colony numbers from 20,000 BMMCs are shown. The size of the colonies was also measured on day 7. Colony morphology was scored on the basis of Stemcell Technologies criteria. L-butionine-sulfoxamine (BSO, Sigma-Aldrich) was added to methylcellulose media at the indicated concentrations at the time of plating.

Zhu H., Kwak H.J., Liu P., Bajrami B., Xu Y., Park S.Y., Nombela-Arrieta C., Mondal S., Kambara H., Yu H., Chai L., Silberstein L.E., Cheng T, & Luo H.R. (2017). Reactive oxygen species-producing myeloid cells act as a bone marrow niche for sterile inflammation-induced reactive granulopoiesis. Journal of immunology (Baltimore, Md. : 1950), 198(7), 2854-2864.

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