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Borrelidin

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Borrelidin is a chemical compound produced by the bacterium Streptomyces rochei. It functions as a potent inhibitor of the enzyme threonine tRNA synthetase, which plays a role in protein synthesis. The core function of Borrelidin is to interfere with the biological processes related to threonine tRNA synthetase.

Automatically generated - may contain errors

Lab products found in correlation

Amv reverse transcriptase, by Roche (2 mentions) Dna ligase, by Thermo Fisher Scientific (2 mentions) Accuprime polymerase, by Thermo Fisher Scientific (2 mentions) Mupirocin, by Merck Group (2 mentions)

2 protocols using borrelidin

1

Antibiotics Translation Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
ERY was purchased from SIGMA (E5389), TEL was provided by Cempra Pharmaceuticals, RU69874 was provided by Roussel Uclaf, borrelidin was from Santa Cruz Biotechnologies (SC200379A), and mupirocin from Sigma (M7694). Antibiotics were dissolved in ethanol and when present in the in vitro translation assays were dried at the bottom of the reaction tube prior to addition of the rest of the components. AMV reverse transcriptase was from Roche (Cat No.10109118001). AccuPrime polymerase (Cat No.12344032) for PCR reactions, restriction enzymes, DNA ligase, and all other chemicals were from Thermo Fisher.
DNA oligonucleotides used in this work are listed in Supplementary Table 1.
Gupta P., Liu B., Klepacki D., Gupta V., Schulten K., Mankin A.S, & Vázquez-Laslop N. (2016). Nascent peptide assists the ribosome in recognizing chemically distinct small molecules. Nature chemical biology, 12(3), 153-158.
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2

Antibiotics Translation Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
ERY was purchased from SIGMA (E5389), TEL was provided by Cempra Pharmaceuticals, RU69874 was provided by Roussel Uclaf, borrelidin was from Santa Cruz Biotechnologies (SC200379A), and mupirocin from Sigma (M7694). Antibiotics were dissolved in ethanol and when present in the in vitro translation assays were dried at the bottom of the reaction tube prior to addition of the rest of the components. AMV reverse transcriptase was from Roche (Cat No.10109118001). AccuPrime polymerase (Cat No.12344032) for PCR reactions, restriction enzymes, DNA ligase, and all other chemicals were from Thermo Fisher.
DNA oligonucleotides used in this work are listed in Supplementary Table 1.
Gupta P., Liu B., Klepacki D., Gupta V., Schulten K., Mankin A.S, & Vázquez-Laslop N. (2016). Nascent peptide assists the ribosome in recognizing chemically distinct small molecules. Nature chemical biology, 12(3), 153-158.
+ Open protocol
+ Expand

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