Chemicals and reagents. GPZ (Generics Ltd, UK), cremophor RH 40 (BASF, Germany), coconut oil (purified, Nigeria), Ethanol, acetonitrile, Sodium chloride, Potassium chloride, Disodium hydrogen phosphate dihydrate, Potassium dihydrogen phosphate, Sodium azide, Propylene glycol, Sodium hydroxide and trypsin (Sigma-Aldrich, USA). All other chemicals and reagents were purchased from Sigma-Aldrich (USA) and were used as received.
Disodium hydrogen phosphate dihydrate
Manufactured by Merck Group
Sourced in Germany, United States, France, Italy
Disodium hydrogen phosphate dihydrate is a chemical compound with the formula Na2HPO4 · 2H2O. It is a white, crystalline solid that is commonly used as a buffer in various laboratory applications. The compound has the ability to maintain a relatively stable pH in aqueous solutions, making it a useful tool for maintaining specific pH conditions in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (17 mentions)
Sodium dihydrogen phosphate monohydrate, by Merck Group (13 mentions)
Sodium hydroxide (naoh), by Merck Group (11 mentions)
Sodium dihydrogen phosphate dihydrate, by Merck Group (10 mentions)
Potassium dihydrogen phosphate, by Merck Group (8 mentions)
Formic acid, by Merck Group (8 mentions)
Hydrochloric acid (hcl), by Merck Group (8 mentions)
Ethanol, by Merck Group (7 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (7 mentions)
Potassium chloride (kcl), by Merck Group (6 mentions)
Phosphoric acid, by Merck Group (5 mentions)
Methanol, by Merck Group (5 mentions)
Acetic acid, by Merck Group (5 mentions)
Acetonitrile, by Merck Group (4 mentions)
Trifluoroacetic acid tfa, by Merck Group (4 mentions)
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Trifluoroacetic acid, by Merck Group (4 mentions)
Trypsin, by Merck Group (3 mentions)
Sodium azide, by Merck Group (3 mentions)
Citric acid monohydrate, by Merck Group (3 mentions)
58 protocols using disodium hydrogen phosphate dihydrate
1
Comprehensive Chemical Reagents Inventory
2
Evaluating Bevacizumab Effects on mIDH1-U87 Cells
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The mIDH1-U87 (U87 cell line stably transfected with IDH1-R132H with a lentiviral vector, and was kindly provided by Marc Sanson [Hôpital de la Salpêtrière, Paris, France]) was maintained in Eagle’s minimal essential medium (EMEM) with 10% foetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin (Lonza, Verviers, Belgium). bevacizumab (Roche, Paris, France) was diluted with culture medium to working concentrations before use. As a control, a stock solution containing the corresponding excipient was prepared with 60 mg/mL of trehalose dehydrate, 5.8 mg/mL sodium dihydrogen phosphate monohydrate, and 1.5 mg di-sodium hydrogen phosphate dihydrate (all from Sigma Aldrich, Saint-Quentin Fallavier, France). VEGF secretion was assessed with the Quantikine ELISA kit for human VEGF (R&D Systems, Abingdon, UK), following the manufacturer’s instructions. The cells proliferation was assessed as follows: The mIDH1-U87 cells were seeded into and allowed to attach overnight. Cells (seeded in 24-well plates; 30,000 cells/well) were treated for 72 h with bevacizumab (from 0.1 mg/mL to 1 mg/mL) in triplicate wells. Duplicate experiments were performed. Cell viability was then assessed with the MTT assay following the procedure of Mosmann.13 (link) The percentage of surviving cells is expressed as the ratio of optical density of treated cells versus untreated cells.
3
Preparation of Phosphate Buffer in D2O
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Deuterium oxide 99.9%, sodium dihydrogenphosphate hydrate (NaH2PO4·H2O), disodiumhydrogen phosphate dihydrate (Na2HPO4·H2O2), and 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP) were purchased from Sigma-Aldrich (Milan, Italy). Deuterated EDTA d-16 98% was obtained from Product Cambridge Isotope Laboratories, Inc.
Phosphate buffer solution was prepared as follows: 49.7 mg of sodium dihydrogenphosphate hydrate (0.36 mmol), 202.9 mg of disodium hydrogen phosphate dihydrate (1.14 mmol), and 0.92 mg deuterated EDTA d-16 (0.003 mmol) were dissolved in 10 mL of water. The pH was checked at 7.1. The solution was distributed into 5 mL aliquots and then lyophilized. Each aliquot was dissolved in about 1 mL of D2O and lyophilized again. Finally, the buffer was dissolved in 5 mL of deuterium oxide with 0.002% TSP (12 mM).
Phosphate buffer solution was prepared as follows: 49.7 mg of sodium dihydrogenphosphate hydrate (0.36 mmol), 202.9 mg of disodium hydrogen phosphate dihydrate (1.14 mmol), and 0.92 mg deuterated EDTA d-16 (0.003 mmol) were dissolved in 10 mL of water. The pH was checked at 7.1. The solution was distributed into 5 mL aliquots and then lyophilized. Each aliquot was dissolved in about 1 mL of D2O and lyophilized again. Finally, the buffer was dissolved in 5 mL of deuterium oxide with 0.002% TSP (12 mM).
4
Oxime K048 Acetylcholinesterase Inhibition
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Oxime K048 (1-(4-hydroxyiminomethylpyridinium)-4-(4-carbamoylpyridinium) butane dibromide) (Mr=460.16 g mol -1 ) was a gift from Dr Kamil Kuča, University of Hradec Kralove, Czech Republic. It was kept at room temperature and dissolved in water or atropine immediately before use. Tabun (ethyl N,N-dimethyl phosphoroamidocyanidate) was purchased from NC Laboratory, Spiez, Switzerland. A stock solution of 5,000 μg mL -1 was prepared in isopropyl alcohol. Further dilutions were made in saline immediately before use. Atropine sulphate (Kemika, Zagreb, Croatia) was dissolved in water. Non-ionic detergent 1 % Triton X-100 was purchased from SERVA Electrophoresis GmbH, Heidelberg, Germany. Acetylthiocholine iodide (ATCh), DTNB (5,5'-dithiobis(2-nitrobenzoic acid)), BW284C51 (1,5-bis(4-allyldimethyl-ammoniumphenyl)-pentane-3-one dibromide), ethopropazine ((10-[2-diethylaminopropyl] phenothiazine)hydrochloride), and chemicals for high performance liquid chromatography (HPLC) [acetonitrile gradient grade LiChrosolv, disodium hydrogen phosphate dihydrate, citric acid monohydrate, ethylenedinitrilo tetraacetic acid disodium salt dihydrate (Na 2 EDTA), 1-octane sulphonic acid sodium salt, perchloric acid (70 %), and phosphoric acid] were purchased from Sigma-Aldrich (St. Louis, MO, USA). The water used for HPLC was double-distilled and deionised HPLC-grade.
5
Synthesis and Characterization of Gold Nanoparticles
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Sodium borohydride (NaBH4), [(1-methyl-1H-imidazol-2-yl)sulfanyl]acetic
acid (IL), and PVP were purchased from Sigma-Aldrich (St. Louis, Missouri,
USA). Tetrachloroauric(III) acid (HAuCl4, 99.99% pure-trace
metal basis, 30% wt in dilute HCl) was purchased from Sigma-Aldrich
(St. Louis, Missouri, USA). Disodium hydrogen phosphate dihydrate
(Na2HPO4·2H2O) and sodium dihydrogen
phosphate dihydrate (NaH2PO4·2H2O) were obtained from Sigma-Aldrich (St. Louis, Missouri, USA). AP
was kindly donated by ROKETSAN Corporation (i.e.,
an establishment of Turkish Armed Forces Foundation). The explosive
materials 2,4,6-trinitrotoluene (TNT), 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane
(HMX), 1,3,5-trinitro-1,3,5-triazacyclohexane (RDX), 2,4,6-trinitrophenyl-N-methylnitramine (tetryl), and pentaerythritoltetranitrate
(PETN) were kindly supplied by the Mechanical and Chemical Industry
Corporation (Makine Kimya Endustrisi Kurumu, MKEK) of Turkey from
previous projects. Picric acid was purchased from Sigma-Aldrich.
acid (IL), and PVP were purchased from Sigma-Aldrich (St. Louis, Missouri,
USA). Tetrachloroauric(III) acid (HAuCl4, 99.99% pure-trace
metal basis, 30% wt in dilute HCl) was purchased from Sigma-Aldrich
(St. Louis, Missouri, USA). Disodium hydrogen phosphate dihydrate
(Na2HPO4·2H2O) and sodium dihydrogen
phosphate dihydrate (NaH2PO4·2H2O) were obtained from Sigma-Aldrich (St. Louis, Missouri, USA). AP
was kindly donated by ROKETSAN Corporation (i.e.,
an establishment of Turkish Armed Forces Foundation). The explosive
materials 2,4,6-trinitrotoluene (TNT), 1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane
(HMX), 1,3,5-trinitro-1,3,5-triazacyclohexane (RDX), 2,4,6-trinitrophenyl-N-methylnitramine (tetryl), and pentaerythritoltetranitrate
(PETN) were kindly supplied by the Mechanical and Chemical Industry
Corporation (Makine Kimya Endustrisi Kurumu, MKEK) of Turkey from
previous projects. Picric acid was purchased from Sigma-Aldrich.
6
Polymer Precursor Synthesis Protocol
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Polyacrylonitrile (PAN, MW-150000), Disodium hydrogen phosphate dihydrate (Na2HPO4.2H2O), Silver nitrate (AgNO3), Sodium sulfate (Na2SO4), were purchased from Sigma-Aldrich, USA. N, N-dimethylformamide (DMF) purchased from Daejung Chemicals, South Korea. All the chemicals were used as received without further purification.
7
Analytical Grade Reagents for Research
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Methanol, acetonitrile and water were of mass spectrometry grade and were obtained from Merck Sigma-Aldrich (Darmstadt, Germany). Trolox reagent, potassium persulfate, sodium chloride, formic acid, sodium dihydrogen phosphate dihydrate, di-sodium hydrogen phosphate dihydrate and the Folin-Ciocalteu′s reagent was purchased from Merck Sigma-Aldrich. All the other chemicals were of analytical grade and were obtained from Merck Sigma-Aldrich unless otherwise indicated.
8
Cytotoxicity Assay for Cell Viability
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HSP, HAuCl4·3H2O, XA, and di-sodium hydrogen phosphate dihydrate were purchased from Sigma-Aldrich. Dimethyl sulfoxide (DMSO), MTT, trypsin, ethanol, EDTA, and phosphate-buffered saline (PBS) were purchased from Sigma Chemicals. All water solutions were prepared from high-purity water filtered with Milli-Q Plus equipment (Millipore Co.). The solvents and analytical chemicals used were highly pure and were not further processed. Cells are purchased from National Centre for Cell Science (NCCS, Pune, India).
9
Lipid Reagents for Membrane Studies
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Diphosphatidyl glycerol (cardiolipin, CL), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), cholesterol (Chol), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium salt, DOPE-CF) (all from Avanti polar lipids, Alabaster, AL, USA) were used as received (the structures are presented in Table 3 ). Disodium hydrogen phosphate dihydrate (Na2HPO4⋅2H2O), sodium dihydrogen phosphate dihydrate (NaH2PO4⋅2H2O), and sodium chloride (all chemically pure grade and were purchased from Sigma-Aldrich, Darmstadt, Germany) were used as received. 10–3 M buffer solution was prepared by weighing.
Double-distilled water was used for making solutions after additional treatment with a Milli-Q (Merck Millipore, Darmstadt, Germany) system that included ion exchange adsorption columns for advanced purification from organic impurities and filters for removing large particles. The conductivity of the purified water was 0.5 μS/cm.
Double-distilled water was used for making solutions after additional treatment with a Milli-Q (Merck Millipore, Darmstadt, Germany) system that included ion exchange adsorption columns for advanced purification from organic impurities and filters for removing large particles. The conductivity of the purified water was 0.5 μS/cm.
10
Characterization of Anti-HER2 Antibody Interactions
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Herceptin (trastuzumab) was from Roche (Basel, Switzerland) and Kadcyla (ado-trastuzumab emtansine (T-DM1)) from Genentech Inc. (South San Francisco, CA, USA). Human HER2/ErbB2s protein (His tagged) was obtained from Sino Biologicals (North Wales, USA). Ethylenediaminetetraacetic acid (EDTA), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), Tween 20, disodium hydrogen phosphate dihydrate, phosphate-buffered saline (PBS) tablets, ethanolamine hydrochloride, N-hydroxy succinimide (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), hydrochloric acid 37% (HCl), acetic acid (≥ 99%), glycine-HCl, ammonium acetate, sodium hydroxide (NaOH), sodium chloride (NaCl), and sodium acetate were purchased from Sigma-Aldrich (Steinheim, Germany). Sodium azide (toxic, should be discarded in hazardous waste) was obtained from Mallinckrodt Baker B.V. (Deventer, the Netherlands). β-mercaptoethanol was obtained from Merck (Darmstadt, Germany). Deionized water was produced by a Milli-Q purification system from Millipore (Amsterdam, the Netherlands).
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