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2 protocols using ciprofibrate

1

Evaluating PPAR Activation Pathways

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Ciprofibrate and rosiglitazone 1, were obtained from Cayman Chemical (Ann Arbor, MI). Dulbecco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS) and phosphate-buffered saline (PBS) were purchased from Hyclone (South Logan, Utah). Penicillin/streptomycin and trypsin were procured from Gibco (Grand Island, NY). Specific plasmids pSG5–PPARα (plasmid 22751) and PPRE X3-tk-luc (plasmid 1015) were obtained from Addgene (Cambridge, MA). pCMV-rPPARγ and pPPREaP2-tk-luc were provided by Dr. Dennis Feller (Department of Pharmacology, University of Mississippi).
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2

Adipocyte Differentiation Assay Reagents

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Ciprofibrate, rosiglitazone, T0901317, 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG) and Hoechst 33342 were purchased from Cayman chemical (Ann Arbor, MI, USA). Oil red O, 3-Isobutyl-1-methylxanthine (IBMX), insulin and dexamethasone were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Hyclone Lab, Inc. (Logan, UT, USA). Phosphate-buffered saline (PBS) (pH 7.4), Hanks balanced salt solution (HBSS), minimal essential medium (MEM), Dulbecco’s modified Eagle medium (DMEM), DMEM/F12, trypsin EDTA, sodium pyruvate, HEPES, streptomycin, and penicillin-G were from GIBCO BRL (Invitrogen Corp., Grand Island, NY, USA).
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