The largest database of trusted experimental protocols

4 protocols using 2 4 6 tris 1 pyridyl 5 triazine

1

Phytochemical Quantification and Antioxidant Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Folin-Ciocalteu's (FC) reagent, methanol, ethanol, sulfuric acid, sodium carbonate, sodium nitrite, sodium hydroxide, sodium acetate, acetic acid, aluminium chloride, potassium peroxodisulphate, and iron (III) chloride were purchased from R & M Chemicals (Essex, UK). 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′azinobis-(3-ethylben-zothiazoline-6-sulfonicacid) (ABTS), 2,4,6-tris(1-pyridyl)-5-triazine (TPTZ), polyvinyl polypyrrolidone (PVPP) and 1,10phenanthrolin, were purchased from Sigma-Aldrich. Gallic acid was supplied by Pleon partners GmbH (Turkey). All chemicals and reagents used in the study were analytical grade.
+ Open protocol
+ Expand
2

Screening Endothelial Cell Viability and Angiogenic Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was purchased from Affymetrix, USA. C. bombicola 22214 and EA.hy926 were obtained from ATCC, USA. Calbiochem, USA, and Corning, USA, supplied fibrinogen and matrigel, respectively. Dulbecco's Modified Eagle's Medium Powder and Medium 199 were purchased from Gibco, Life Technology, UK. Yeast extract and yeast malt agar were purchased from HiMedia, India. Ascorbic acid, Folin–Ciocalteu reagent, gallic acid, hydrochloric acid, and methanol were acquired from Merck, Germany. Solvents were purchased from QReC, New Zealand. Raybio, USA supplied human vascular endothelial growth factor (VEGF) Assay Kit. Acetic acid, ferrous sulfate, sodium acetate trihydrate, and sodium carbonate were obtained from R and M Chemicals, Essex, UK. 2,2'-azinobis (3-ethylbenzthiazolinesulfonic acid), 2,4,6-tris(1-pyridyl)-5-triazine (TPTZ), aprotinin, CaCl2, C6H12O6, crystal violet, 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ɛ-aminocaproic acid, KH2PO4, L-glutamine, MgSO4.7H2O, Na2CO3, NaCl, Na2SO4, potassium persulfate, suramin, and thrombin 1000 unit were purchased from Sigma-Aldrich, Germany.
+ Open protocol
+ Expand
3

Antioxidant and Antibacterial Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
All solvents used in this study were of high-performance liquid chromatography (HPLC) grade. Trifluoroacetic acid (extra-pure grade) was supplied by Alfa Aesar (Ward Hill, MA, USA). Quercetin-3,4′-O-diglucoside and quercetin-4′-O-monoglucoside were supplied by Polyphenols Laboratories AS (Sandnes, Norway). Gallic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-Tris (1-pyridyl)-5-triazine (TPTZ), ferric chloride, Folin Ciocalteu reagent and ampicillin used were purchased fromSigma–Aldrich (St. Louis, MO, USA), and 2,2-diphenyl-1-picrylhydrazyl from Wako Pure Chemicals (Osaka, Japan) were used for antioxidant assays. Stock solutions of quercetin (1 mg mL−1) and quercetin glucosides (4 mg mL−1) were prepared in methanol. All the solutions were stored at −20 °C and calibration standards were obtained by appropriate dilution of the stock solutions. The standard bacterial strains of the Gram negative bacteria E. coli (KCTC-1924), Pseudomonas aeruginosa (KCTC-2004), and of the Gram positive bacteria B. cereus (KCTC-1012) and Staphylococcus aureus (KCTC-1916) were obtained from the Korean Collection for Type Cultures (KCTC).
+ Open protocol
+ Expand
4

Antioxidant and Cytotoxic Potential Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,4,6-tris(1-pyridyl)-5-triazine (TPTZ), 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS), poloxamer 407, quercetin, morin, and potassium persulfate were purchased from Sigma (St Louis, MO, USA). Folin–Ciocalteu reagent, methanol, gallic acid, and l-ascorbic acid were obtained from Merck (Darmstadt, Germany). Sodium carbonate, sodium acetate trihydrate, aluminum chloride, ferric chloride hexahydrate, carboxymethyl cellulose (CMC), and ferrous sulfate heptahydrate were purchased from R&M Chemicals (Essex, UK). Atorvastatin was obtained from Ranbaxy Malaysia Sdn. Bhd. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide was purchased from Sigma (St Louis, MO, USA). Normal cell line – human umbilical vein cell line (EA.hy926) and cancer cell lines – human colon cancer cell line (HCT116), human colon cancer cell line (HT29), and human cervical cancer cell line (HeLa) are kind gift from the Oncology and Radiology Science Cluster, Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!