Immunoglobulin G (IgG) reactivity in sera was determined by ELISA. Briefly, Nunc Maxisorp plates (Roskilde, Denmark) were coated overnight with 10 μg/ml of GAS M protein antigen (rM5) or heat denatured rat cardiac myosin. After blocking, rat sera were applied in triplicate at 1:100 dilution for rM5 and 1:4 dilution for cardiac myosin for 1 h at 37 °C. Secondary antibody goat anti-rat IgG (H+L) (Jackson Immunoresearch, West Grove, PA) conjugated with horseradish-peroxidase was added for 1 h at 37 °C. After washing, 2-2′–azino-di(3-ethylbenzthiazoline)-6-sulphonate (ABTS) was added for 30 min before OD was measured at 405 nm. Controls included a positive serum sample, normal rat serum as negative control and antibody conjugate alone.
Goat anti rat igg h l
Manufactured by Jackson ImmunoResearch
Sourced in United States
Goat anti-rat IgG (H+L) is a secondary antibody produced by immunizing goats with rat immunoglobulin G (IgG). It recognizes both the heavy (H) and light (L) chains of rat IgG, providing a versatile tool for various immunoassays and detection applications.
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Lab products found in correlation
Goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions)
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γ1 actin 2f3, by Fujifilm (1 mentions)
Peroxidase conjugated goat anti mouse igg h l, by Jackson ImmunoResearch (1 mentions)
Cytochrome c 7h8.2c12, by BD (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using goat anti rat igg h l
1
Quantifying Antibody Response to GAS M Protein
2
Antibody Immunoblotting Protocol
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Mouse monoclonal antibodies to FLAG (IE6; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), VSV-G (P5D4; Santa Cruz Biotechnology, Dallas, TX, USA), the VSV glycoprotein (P5D4, SAB4200695; Sigma-Aldrich), GRP75 (D-9; Santa Cruz Biotechnology), cytochrome c (7H8.2C12; BD Biosciences, Franklin Lakes, NJ, USA), PARP-1 (C2-10; R &D Systems, Minneapolis, MN, USA), γ1-actin (2F3; FUJIFILM Wako Pure Chemical Corporation), β-actin (AC-15; Sigma-Aldrich), and GAPDH (6C5; Santa Cruz Biotechnology), a rat monoclonal antibody to caspase-7 (11E4; Sigma-Aldrich), and a rabbit polyclonal antibody to HSP60 (insect, ADI-SPA-805; Enzo Life Sciences, Farmingdale, NY, USA) were used as primary antibodies. Peroxidase-conjugated goat anti-mouse IgG (H + L) (115-035-146; Jackson ImmunoResearch Laboratories, West Grove, PA, USA), goat anti-rabbit IgG (H + L) (111-035-144; Jackson ImmunoResearch Laboratories), and goat anti-rat IgG (H + L) (J112-035-143; Jackson ImmunoResearch Laboratories) were used as secondary antibodies.
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