Cy5 conjugated goat anti rabbit

Manufactured by Jackson ImmunoResearch

Sourced in Panama, Sweden

Cy5-conjugated goat anti-rabbit is a secondary antibody that is labeled with the fluorescent dye Cyanine 5 (Cy5). This antibody is designed to bind to and detect rabbit primary antibodies in various immunoassays and applications.

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Lab products found in correlation

Cy3 conjugated goat anti rabbit, by Jackson ImmunoResearch (3 mentions) Cy3 conjugated goat anti mouse, by Jackson ImmunoResearch (3 mentions) Cy3 conjugated streptavidin, by Jackson ImmunoResearch (2 mentions) Rabbit anti parvalbumin, by Swant (2 mentions) Mab377, by Merck Group (1 mentions) Ab9116, by Abcam (1 mentions) Mouse anti β tubulin, by Merck Group (1 mentions) Cy2 conjugated goat anti mouse, by Jackson ImmunoResearch (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cy3 conjugated goat anti rat, by Jackson ImmunoResearch (1 mentions) Cy3 conjugated goat anti rabbit, by Merck Group (1 mentions) Anti phospho jnk, by Cell Signaling Technology (1 mentions) Streptavidin alexa 488, by Thermo Fisher Scientific (1 mentions) Rabbit polyclonal anti iba1, by Fujifilm (1 mentions) Goat anti chicken alexa 488, by Thermo Fisher Scientific (1 mentions) Chicken polyclonal anti gfp, by Abcam (1 mentions) Biotinylated goat anti chicken, by Vector Laboratories (1 mentions) Cy3 conjugated donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Sc 216, by Santa Cruz Biotechnology (1 mentions) A1r mp, by Nikon (1 mentions)

Close spelling variants of this product

Anti-rabbit Cy5 (28 citations)

6 protocols using cy5 conjugated goat anti rabbit

Immunohistochemistry of Synuclein and Neurotransmitters

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The following primary antibodies were used: mouse anti-α-Syn (BD610787, BD Biosciences, Franklin Lakes, NJ), rabbit anti-V5 (ab9116, Abcam, Cambridge, UK), mouse anti-α-Syn (LB509, Covance, Munich, Germany), rabbit anti-TH (Zytomed, Berlin, Germany), rabbit anti-TPH2 (ABN60, Millipore, Darmstadt, Germany), mouse anti-NeuN (MAB377, Millipore) and mouse anti-β-tubulin (Sigma, St- Louis, MO).
The following secondary antibodies were used: Cy2-conjugated goat anti-mouse, Cy3-conjugated goat anti-rabbit, Cy3-conjugated goat anti-mouse, Cy5-conjugated goat anti-rabbit (all from Jackson Immuno Research, West Grove, PA); horseradish peroxidase(HRP)-coupled goat anti-mouse and HRP-coupled goat anti-rabbit (both from Dianova, Hamburg, Germany).

Tatenhorst L., Eckermann K., Dambeck V., Fonseca-Ornelas L., Walle H., Lopes da Fonseca T., Koch J.C., Becker S., Tönges L., Bähr M., Outeiro T.F., Zweckstetter M, & Lingor P. (2016). Fasudil attenuates aggregation of α-synuclein in models of Parkinson’s disease. Acta Neuropathologica Communications, 4, 39.

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Imaging of Drosophila Larval Wing Discs

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Larval tissues were collected and treated at 110–120 hours after egg deposition. Larvae were dissected in 1xPBS at room temperature and fixed for 20 minutes in 4% formaldehyde and the immunostaining procedure was performed as previously described^{35 (link)}. After several washes in 1xPBS+0,3% Triton X-100, wing imaginal discs were mounted on microscopy slides with Fluoromount G. Subsequently, samples were analysed with TCS SL Leica confocal system. Digital images were assembled using the Adobe Photoshop software. The following primary antibodies were used: monoclonal mouse anti-ci 1:50 (DSHB) and anti-phosphoJNK 1:400 (Cell Signaling Technology) were detected with Cy3-conjugated goat anti-mouse 1:500 (Jackson); polyclonal rabbit anti-Awd^{12 (link)} 1:1000 was detected using Cy3-conjugated goat anti-rabbit 1:1000 (Jackson) or DyLight 647-conjugated goat anti-rabbit 1:500 (Jackson); rabbit anti-MMP1 1:50 (DSHB) was detected using Cy3-conjugated goat anti-rabbit 1:1000 (Jackson); anti-cleaved-Caspase3 1:100 (Cell Signaling Technology) was detected using Cy3-conjugated goat anti-rabbit 1:2000 (Sigma); anti-ξPKC 1:200 (Santa Cruz Biotechnology) was detected with Cy5-conjugated goat anti-rabbit 1:1000 (Jackson). DE-Cad 1:25 (DSHB) was detected using Cy3-conjugated goat anti-rat 1:1000 (Jackson).

Romani P., Duchi S., Gargiulo G, & Cavaliere V. (2017). Evidence for a novel function of Awd in maintenance of genomic stability. Scientific Reports, 7, 16820.

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Verifying GFP-labeled Neurons and Parvalbumin Expression

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Triple immunofluorescence stainings of biocytin-filled neurons were performed to verify that recordings were obtained from GFP-labeled neurons and to examine parvalbumin expression. Slices were fixed overnight with 4% paraformaldehyde, rinsed with PBS and treated for 90 min with PBS containing 10% normal goat serum, 0.2% Triton X-100, 20% avidin (block A, blocking kit, Vector, USA). Subsequently slices were incubated overnight with the primary antibody rabbit anti-parvalbumin (1∶1000, Swant, Switzerland) diluted in PBS containing 1% normal goat serum, 0.2% Triton X-100, 20% biotin (block B, blocking kit, Vector, USA). Neurons were analyzed on the following day after incubating slices for 90 min with Cy5-conjugated goat anti-rabbit (1∶250, Jackson ImmunoResearch Europe) together with streptavidin-conjugated Cy3 (1∶250, Jackson ImmunoResearch Europe).

Imbrosci B., Neitz A, & Mittmann T. (2014). Focal Cortical Lesions Induce Bidirectional Changes in the Excitability of Fast Spiking and Non Fast Spiking Cortical Interneurons. PLoS ONE, 9(10), e111105.

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Visualizing Parvalbumin Interneurons and Biocytin-Filled Neurons

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Slices containing biocytin-filled neurons were fixed overnight with 4% paraformaldehyde and rinsed in PBS. For detection of parvalbumin-positive interneurons some slices were treated for 90 min with PBS containing 10% normal goat serum, 0.2% Triton X-100, and 20% avidin (block A, blocking kit, Vector, USA). Subsequently slices were incubated overnight with the primary antibody rabbit anti-parvalbumin (1 : 1000, Swant, Switzerland) diluted in PBS containing 1% normal goat serum, 0.2% Triton X-100, 20% biotin (block B, blocking kit, Vector, USA). The following day parvalbumin-expressing neurons were visualized by incubating slices for 90 min with Cy5-conjugated goat anti-rabbit (1 : 250, Jackson ImmunoResearch Europe) together with streptavidin-conjugated Cy3 (1 : 250, Jackson ImmunoResearch Europe) for the visualization of biocytin-filled neurons. In slices where parvalbumin expression was not detected the incubation with anti-parvalbumin antibody and the following treatment with Cy5-conjugated goat anti-rabbit were omitted. The size of the neuronal soma was measured semiautomatically with the software Image J (National Institutes of Health, USA).

Imbrosci B., Neitz A, & Mittmann T. (2015). Physiological Properties of Supragranular Cortical Inhibitory Interneurons Expressing Retrograde Persistent Firing. Neural Plasticity, 2015, 608141.

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Confocal Microscopy of Retrovirally Labeled Neurons

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For confocal microscopy, rats were perfused with ice-cold saline and 4% paraformaldehyde 3 or 6 weeks after retroviral injections. Brains were processed for immunohistochemistry as previously described [11 (link)]. The following primary and secondary antibodies were used: rabbit polyclonal anti-Iba1 (1:1000, Wako, Japan), chicken polyclonal anti-GFP (1:5000, Abcam, UK), mouse monoclonal anti-gephyrin (1:500, Synaptic Systems, Germany), Cy3-conjugated donkey anti-rabbit (1:200, Jackson Immunoresearch, UK), Cy3-conjugated goat anti-mouse (1:200, Jackson Immunoresearch), biotinylated goat anti-chicken (Vector laboratories), Cy5-conjugated goat anti-rabbit (1:200, Jackson Immunoresearch), Alexa-488 goat anti-chicken (1:200, Invitrogen, Sweden), and Alexa-488 streptavidin (1:200, Invitrogen).

Chugh D, & Ekdahl C.T. (2016). Interactions Between Microglia and Newly Formed Hippocampal Neurons in Physiological and Seizure-Induced Inflammatory Environment. Brain Plasticity, 1(2), 215-221.

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Immunostaining of Drosophila Larval Brains

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Larval brain staining was performed as previously described [18] (link). The following primary antibodies were used: guinea pig anti-Deadpan (1∶1000, Luo lab), rat anti-Miranda (1∶1000, a gift from Chris Doe), guinea pig anti-Numb (1∶1000, a gift from James Skeath), rabbit anti-activated caspase-3 (9661S, 1∶200, Cell Signaling Technology), rabbit anti-pAkt (D9E, 1∶100, Cell Signaling Technology), rabbit anti-phospho-Histone H3 (06-570, 1∶500, Upstate Biotechnology), mouse anti-Discs large [4F3, 1∶100, Developmental Studies Hybridoma Bank (DSHB)], mouse anti-Dachshund (mAbdac2-3, 1∶100, DSHB), rat anti-Elav (7E8A10, 1∶100, DSHB), mouse anti-β-Tubulin (E7, 1∶20, DSHB), rabbit anti-DE-Cadherin (sc-33743, 1∶100, Santa Cruz Biotechnology), rabbit anti-aPKC (sc-216, 1∶1000, Santa Cruz). The secondary antibodies used were: Alexa Fluo-488 goat anti-rabbit (1∶200, Molecular Probes); Cy3-conjugated donkey anti-mouse (1∶200), Cy3-conjugated goat anti-rabbit (1∶200), Cy5-conjugated donkey anti-rat (1∶200), Cy5-conjugated goat anti-rabbit (1∶200) and Cy5-conjugated donkey anti-guinea pig (1∶200) (Jackson ImmunoResearch Lab).
Confocal images were obtained by Olympus FV500 (60 x objective, N.A.1.4) and Nikon A1R MP (60 x (WI) objective, N.A.1.27) confocal microscopes, and processed with Imaris (Bitplane) and Adobe Photoshop CS software.

Chen X., Quan Y., Wang H, & Luo H. (2014). Trehalase Regulates Neuroepithelial Stem Cell Maintenance and Differentiation in the Drosophila Optic Lobe. PLoS ONE, 9(7), e101433.

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