Ascorbic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH●), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS●+), neocuproine, trichloroacetic acid (TCA), and potassium ferricyanide were used for the determination of the antioxidant activities and were purchased from Sigma Chemical Co. (Sigma-Aldrich GmbH, Stern-heim, Germany). The following chemicals were used for the enzyme inhibition assays: α-glucosidase from Saccharomyces cerevisiae, 4-nitrophenyl α-D-glucopyranoside (pNPG), α-amylase from porcine pancreas, β-nicotinamide adenine dinucleotide (β-NADH), phenazinemethosulphate (PMS), nitrotetrazolium blue chloride (NBT) and Ascorbic acid were obtained from Sigma (St. Louis, MO, USA), while acarbose and potato starch were purchased from Fluka (Bucharest, Romania) and Fisher (Pittsburgh, PA, USA), respectively. Sodium nitroprusside, sulfanilamide, and 3,5-dinitrosalicylic acid (DNS) were obtained from Acros Organics (Hampton, NH, USA). Folin–Ciocalteu reagent, Na2CO3, and gallic acid were purchased from Panreac (Barcelona, Spain). Standards used for the elucidation of the phenolic compounds and for elaboration of the calibration curves were obtained from EXTRA SYNTHESE (Genay Cedex, France). Acetonitrile HPLC-grade and formic acid were purchased from Panreac (Barcelona, Spain). All other chemicals were of analytical grade.
Hplc grade acetonitrile
Manufactured by ITW Reagents
Sourced in Spain, United States
HPLC-grade acetonitrile is a high-purity solvent used in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic odor. The high purity and low impurity levels of this solvent make it suitable for use in HPLC analysis and separation techniques.
Automatically generated - may contain errors
Lab products found in correlation
Milli q system, by Merck Group (4 mentions)
Methanol, by ITW Reagents (4 mentions)
Quercetin, by Merck Group (4 mentions)
Formic acid, by ITW Reagents (3 mentions)
Formic acid, by Merck Group (3 mentions)
Gallic acid, by Merck Group (3 mentions)
Potassium ferricyanide, by Merck Group (2 mentions)
Folin ciocalteu reagent, by ITW Reagents (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Milli q purification system, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
Chlorogenic acid, by Merck Group (2 mentions)
Ferulic acid, by Merck Group (2 mentions)
Kaempferol, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by RCI Labscan (2 mentions)
Naringin, by Merck Group (2 mentions)
Methanol, by RCI Labscan (2 mentions)
Myricetin, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by ITW Reagents (2 mentions)
22 protocols using hplc grade acetonitrile
1
Bioactive Compound Extraction and Evaluation
2
Quantification of NAD+ via HPLC
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β-nicotinamide adenine dinucleotide, acetonitrile (HPLC grade) and Triton X-100 were purchased from PanReac AppliChem, Halt Protease Inhibitor Cocktail (100X) and 16% methanol-free formaldehyde from Thermo Fisher Scientific, TriReagent from MRC. Alda-1 was synthesized by Dr. A.V. Kurkin (Moscow State University, Russia). All other chemicals were from Sigma Aldrich (Vienna, Austria) if not specified otherwise.
3
HPLC-grade Solvent Preparation
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Acetonitrile HPLC-grade was purchased from (PANREAC, Barcelona, Spain), formic acid and KOH were purchased from (Sigma-Aldrich, St. Louis, MO, USA). Ethanol was purchased from (LLC ORT “Khimreaktivy”, Yekaterinburg, Russia). For all experiments we used deionized water (Siemens Ultra-Clear TWF Water Purification Systems, Günzburg, Germany).
4
Reagents and Materials for Biochemical Assays
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Potassium ferricyanide, ferrous sulfate (II), ethylenediaminetetraAcetic acid (EDTA), gallic acid, ammonium molybdate, hydrogen peroxide 30%, Acetic acid, Folin–Ciocalteu phenol reagent, ethanol, and sulfuric acid were obtained from Merck (Darmstadt, Germany). Nitro blue tetrazolium (NBT), monosaccharides, EDTA, ascorbic acid, methionine, bovine serum albumin (BSA), pyrocatechol violet, ascorbic acid, and ammonium molybdate were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Sodium bicarbonate, culture media components (Minimum essential Dulbecco’s modified Eagle medium (DMEM)), non-essential amino acids, fetal bovine serum, sodium pyruvate, and phosphate buffered saline (PBS) were purchased from Invitrogen Canada Inc. (Burlington, ON, Canada). Acetonitrile (HPLC grade) purchased from Panreac® (São Paulo, Brazil). Acetic acid was provided by Vetec® (São Paulo, Brazil). Water was purified with a Milli-Q system (Millipore®, Bedford, MA, USA). All other solvents and chemicals were of analytical grade.
5
Comprehensive Analytical Protocol for Natural Antioxidants
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All the chemicals and reagents were of analytical grade and were supplied by Sigma-Aldrich (St. Louis, MO, USA): Folin–Ciocalteu’s phenol reagent, sodium carbonate, sodium nitrite, aluminum chloride, sodium hydroxide, acetic acid, sodium acetate, 2,4,6,-tri(2pyridyl)-1,3,5,-triazine (TPTZ), iron (III) chloride, 1,1-diphenyl-2-picrylhydrazyl (DPPH), sodium chloride, potassium chloride, dipotassium hydrogen phosphate, disodium hydrogen phosphate, ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), and standards of gallic acid and rutin. Methanol (HPLC grade) was obtained from Honeywell (Morristown, NJ, EEUU), pure acetone (pharma grade), and acetonitrile (HPLC grade) from PanReac AppliChem (Barcelona, Spain). Hydroxytyrosol (98% of purity, w/w) was procured from Extrasynthese (Lyon, France). Ultrapure water was obtained using a Milli-Q system (Millipore, Bedford, MA, USA).
6
Characterization of Synthetic Matrices
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Propyl Paraben (>99% purity), humic acid, sodium chloride, and sodium bicarbonate were supplied from Aldrich. Sodium sulfate was purchased from Scharlau. Acetonitrile HPLC grade was supplied from PanReac AppliChem (Barcelona, Spain). The physico-chemical characterization of the synthetic or environmental matrices investigated is summarized in Table 1 .
7
Bovine Hemin Porphyrin Extraction
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Type I bovine hemin (chloroferriprotoporphyrin IX) and formic acid were obtained from Sigma-Aldrich (USA). Hydroxyapatite and glacial acetic acid was purchased from BDH Chemical Ltd (England). DMSO (99% purity) was purchased from Merck (Germany). Analytical grade methanol was purchased from Fisher Scientific (USA) and acetonitrile HPLC grade was purchased from Panreac (Spain). H2O was deionized using Ultra Clear UV plus TM water system (SG water, Germany) to the quality of 18.2 MOhm.
8
Extraction and Formulation of Jatrophone
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The dichloromethane fraction from J. isabellei (DFJi) was resuspended in a mixture composed of dimethyl sulphoxide (DMSO), polyethylene glycol 400 (PEG 400), and phosphate buffered saline (PBS), pH 7.4 (5:47.5:47.5 v/v) for oral administration (p.o.) in rats. For intravenous administration, the fraction was dissolved in a mixture composed of DMSO, polysorbate 80, and saline solution (5:4:91 v/v). A combination of κ- and λ-carrageenan was purchased from BDH Chemicals Ltd. (London, UK). Dexamethasone and indomethacin were purchased from Deg (São Paulo, Brazil), and colchicine from Sigma-Aldrich (St Louis, MO). The jatrophone isolation from the dichloromethane fraction required the use of Silica Gel 60 and Silica Gel 60 F254-coated plates, which were purchased from Merck (Darmstadt, Germany). Analytical grades hexane, acetone, dichloromethane, ethanol and methanol were purchased from Vetec (Duque de Caxias, Brazil). Acetonitrile HPLC grade was purchased from Panreac (Barcelona, Spain). The ultrapure water utilized in the UFLC analyses was obtained using a Milli-Q purification system (Millipore, Billerica, MA).
9
Nanoparticle Formulation for Targeted Delivery
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Dexamethasone, melatonin and coenzyme Q10 were supplied by Sigma-Aldrich (St.
Louis Mo., USA) at the highest available purity. Poly(D,L-lactide-co-glycolide) (PLGA) 50:50 (Mwt 35,000 g/mol) was purchased from Evonik España (Granollers, Spain).
Polyvinyl alcohol 67,000 g/mol (PVA) was supplied by Merck KGaA (Darmstadt, Germany). Isopropyl alcohol, methanol and acetonitrile, HPLC grade (PanReac AppliChem, Barcelona, Spain) were used to prepare the mobile phases in HPLC determinations. All other chemicals were reagent grade and used as received.
Louis Mo., USA) at the highest available purity. Poly(D,L-lactide-co-glycolide) (PLGA) 50:50 (Mwt 35,000 g/mol) was purchased from Evonik España (Granollers, Spain).
Polyvinyl alcohol 67,000 g/mol (PVA) was supplied by Merck KGaA (Darmstadt, Germany). Isopropyl alcohol, methanol and acetonitrile, HPLC grade (PanReac AppliChem, Barcelona, Spain) were used to prepare the mobile phases in HPLC determinations. All other chemicals were reagent grade and used as received.
10
Carotenoid and Phenolic Compound Analysis
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The methanol, hexane, acetone, petroleum ether, dichloromethane, and hydrochloric acid were of analytical grade and were purchased from Labscan (Dublin, Ireland). The HPLC-grade methanol, HPLC-grade acetonitrile, HPLC–grade ethyl acetate, formic acid, sodium chloride, and potassium hydroxide were obtained from Panreac (Barcelona, Spain). The β-Carotene, all-trans-β-apo-8′-carotenal, α-carotene, phytoene, violaxanthin, lutein, β-cryptoxanthin, and lycopene were purchased from Sigma-Aldrich (Taufkirchen, Germany) and the antheraxanthin from DHI (Hørsholm, Denmark). The lutein epoxide, luteoxanthin, zeinoxanthin, 9-cis-antheraxanthin, 9-cis-violaxanthin, 13-cis-violaxanthin, and 9-cis-lutein were obtained as described elsewhere [23 (link),24 (link),25 (link),26 (link),27 (link)]. The gallic acid, p-hydroxybenzoic acid, syringic acid, caffeic acid, m-coumaric, p-coumaric, chlorogenic acid, ferulic acid, naringin, naringenin, ethyl galate, quercetin, kaempferol, crisin, vanillic acid, and myricetin were purchased from Sigma-Aldrich (Madrid, Spain). The quercitrin was obtained from Extrasynthese (Genay, France). All the aqueous solutions were prepared with purified water in a NANOpure DlamondTM system (Barnsted Inc., Dubuque, IO, USA).
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