1 2 dithio bis nitro benzoic acid dtnb

Sodium nitrite (NaNO₂); sodium carbonate (Na₂CO₃); sodium hydroxide (NaOH); folin-ciocalteu reagent; 2, 2-diphenlyl-1-picrylhydrazyl (DPPH); aluminium chloride hexahydrate (AlCl₃.6H₂O); ferric chloride (FeCl₃); phenazine methosulphate (PMS); nitro blue tetrazolium (NBT); trichloroacetic acid (TCA); thiobarbituric acid (TBA); ammoniummolybdate; hydrogen peroxide (H₂O₂); sodium phosphate; sulphuric acid; ethylenediaminetetraacetic acid (EDTA); 2, 2 azo bis, 3-ethylbenzothiozoline-6-sulphonic acid (ABTS); 2-deoxyribose; ascorbic acid; potassium ferricyanide [K₃Fe (CN)₆]; reduced glutathione (GSH); potassium persulfate (K₂S₂O₈); bovine serum albumin (BSA) and 1,2-dithio-bis nitro benzoic acid (DTNB) were purchased from Sigma Co. (St. Louis, USA). All the solvents i.e. acetonitrile, methanol, acetic acid, dimethylsulfoxide (DMSO), hexane, chloroform, ethyl acetate and butanol were purchased from Sigma-Aldrich, Germany. Chemical standards for quercetin, myricetin, kaempferol, catechin, gallic acid, caffeic acid, rutin and apigenin were of analytical grade.

Doxorubicin, superoxide dismutase (SOD), iron chloride (FeCl₃), nitro blue tetrazolium (NBT), ascorbic acid (AA), catalase (CAT) from bovine liver, acetaminophen (APAP), 2,2′-Diphenyl-1-picrylhydrazyl (DPPH), n-alkanes (C6–C30), thiobarbituric acid (TBA), trichloroacetic acid (TCA), ethylenediaminetetra acetic acid (EDTA), nicotinamide-adenine dinucleotide phosphate (NADPH), butylated hydroxytoluene, gallic acid, sodium carboxymethyl cellulose (CMC), phenazine methosulfate, Folin–Ciocalteu reagent, reduced glutathione (GSH), and 1,2-dithio-bis nitro benzoic acid (DTNB) were purchased from Sigma Co. (St. Louis, MO, USA). Dimethyl sulfoxide (DMSO) was purchased from Merck (Merck, Darmstadt, Germany). Streptomycin, Dulbecco’s modified Eagle’s medium (DMEM), and Streptomycin were purchased from Biochrom (Biochrome AG, Berlin, Germany, A 321-44). Fetal bovine serum was purchased from Sigma (Sigma, Darmstadt, Germany). Potassium phosphate monobasic, sodium pyrophosphate dibasic, sodium carbonate (Na₂CO₃), disodium hydrogen phosphate (Na₂HPO₄), hydrogen peroxide (H₂O₂), potassium ferricyanide (K₃Fe(CN)₆), sodium sulphate anhydrous (Na₂SO₄), acetic acid (ACA), and n-butanol (99.8%) were of analytical grade and purchased from Merck (Nottingham, UK).