Purification
and isolation of compounds were carried out using a preparatory HPLC
from Waters Corp. (Milford, MA, USA) consisting of a W717 plus autosampler,
a W600E multisolvent delivery system, a W600 controller, a W490E multiwavelength
detector, and a Waters fraction collector. A Waters liquid chromatography–mass
spectrometry (LC-MS) Integrity system consisting of a solvent delivery
system with a W616 pump and a W600S controller, a W717 plus autosampler,
a W996 PDA detector, and a Waters TMD Thermabeam electron impact (EI)
single-quadrupole mass detector with fixed ionization energy of 70
eV was used for analysis. Data were collected and analyzed with the
Waters Millennium v. 3.2 software, linked with the sixth edition of
the Wiley Registry of Mass Spectral Data, containing 229,119 EI spectra
of 200,500 compounds.
and isolation of compounds were carried out using a preparatory HPLC
from Waters Corp. (Milford, MA, USA) consisting of a W717 plus autosampler,
a W600E multisolvent delivery system, a W600 controller, a W490E multiwavelength
detector, and a Waters fraction collector. A Waters liquid chromatography–mass
spectrometry (LC-MS) Integrity system consisting of a solvent delivery
system with a W616 pump and a W600S controller, a W717 plus autosampler,
a W996 PDA detector, and a Waters TMD Thermabeam electron impact (EI)
single-quadrupole mass detector with fixed ionization energy of 70
eV was used for analysis. Data were collected and analyzed with the
Waters Millennium v. 3.2 software, linked with the sixth edition of
the Wiley Registry of Mass Spectral Data, containing 229,119 EI spectra
of 200,500 compounds.