Electron microscopy was done to assess ultrastructural changes in the sciatic nerve of mice lacking LXR in sensory neurons. Briefly, mice were deeply anesthetized with isoflurane and transcardially perfused with PBS followed by fixative(2% paraformaldehyde, 2.5% glutaraldehyde, 0.1 M sodium phosphate buffer). Sections of the nerve were obtained using a Leica Ultracut UCT 6 ultramicrotome (Leica Microsystems) and stained with 2% uranyl acetate and lead citrate. Sections were placed on copper grids (Thermo Fisher Scientific) and images were acquired on a Tecnai G2 spirit TEM (FEI).
Ultracut uct 6 ultramicrotome
Manufactured by Leica
The Leica Ultracut UCT 6 is an ultramicrotome used for the preparation of ultra-thin sections for electron microscopy. It is designed to precisely cut samples into thin slices with a thickness of up to 50 nanometers, enabling detailed analysis of microscopic structures.
Automatically generated - may contain errors
4 protocols using ultracut uct 6 ultramicrotome
1
Ultrastructural Analysis of Sciatic Nerve in LXR-Deficient Mice
2
Yeast Cell Ultrastructural Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Yeast cells were grown in the desired conditions and processed in the University of Texas Southwestern Electron Microscopy Core Facility using an adapted protocol from Wright (Wright, 2000 (link)). In brief, cells were fixed in potassium permanganate, dehydrated, stained in uranyl acetate, and embedded in Spurr Resin. Specimen blocks were polymerized at 60°C overnight and sectioned at 70 nm with a diamond knife (Diatome) on a Leica Ultracut UCT 6 ultramicrotome (Leica Microsystems). Sections were poststained with 2% uranyl acetate in water and lead citrate. Sections were placed on copper grids (Thermo Fisher Scientific). Images were acquired on a Tecnai G2 spirit TEM (FEI) equipped with a LaB6 source at 120 kV by using a Gatan Ultrascan charge-coupled device camera.
3
Yeast Cell Ultrastructural Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Yeast cells were grown in the desired conditions and processed in the University of Texas Southwestern Electron Microscopy Core Facility using a protocol adapted from Wright (2000) (link). In brief, cells were fixed in potassium permanganate, dehydrated, and stained in uranyl acetate and embedded in Spurr Resin. Specimen blocks were polymerized at 60°C overnight and sectioned at 70 nm with a diamond knife (Diatome) on a Leica Ultracut UCT 6 ultramicrotome (Leica Microsystems). Sections were poststained with 2% uranyl acetate in water and lead citrate. Sections were placed on copper grids (Thermo Fisher Scientific). Images were acquired on a Tecnai G2 spirit TEM (FEI) equipped with a LaB6 source at 120 kV by using a Gatan Ultrascan charge-coupled device camera.
4
Transmission Electron Microscopy Sample Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed on MatTek dishes with 2.5% (vol/vol) glutaraldehyde in 0.1 M sodium cacodylate buffer. After three washes in 0.1 M sodium cacodylate buffer, cells were postfixed in 1% osmium tetroxide and 0.8% K3[Fe(CN6)] in 0.1 M sodium cacodylate buffer for 1 h at room temperature. Cells were rinsed with water and en bloc stained with 2% aqueous uranyl acetate overnight. After three rinses with water, specimens were dehydrated with increasing concentration of ethanol, infiltrated with Embed-812 resin, and polymerized in a 60°C oven overnight. Blocks were sectioned with a diamond knife (Diatome) on a Leica Ultracut UCT 6 ultramicrotome (Leica Microsystems) and collected onto copper grids, poststained with 2% uranyl acetate in water and lead citrate. Images were acquired on a Tecnai G2 spirit transmission electron microscope (FEI) equipped with an LaB6 source using a voltage of 120 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!