Rabbit immunoglobulin g

Manufactured by Abcam

Rabbit immunoglobulin G (IgG) is a type of antibody purified from rabbit serum. IgG is the most abundant class of antibodies in the body and plays a crucial role in the adaptive immune response.

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Lab products found in correlation

Rabbit anti tbk1, by Cell Signaling Technology (1 mentions) Protein g agarose, by Roche (1 mentions) Protein g magnetic beads, by Active Motif (1 mentions) Ab2743, by Abcam (1 mentions) Chrebp antibody, by Novus Biologicals (1 mentions)

2 protocols using rabbit immunoglobulin g

ASFV-WT Infection and Co-IP Assay

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PAMs were infected with ASFV-WT at an MOI of 1, and the lysates were collected at 48 hpi for the co-IP assay. Protein G agarose (catalog no. 11243233001; Roche) was incubated with the lysates and rabbit anti-pA137R PAbs or a rabbit immunoglobulin G (catalog no. ab190492; Abcam) (isotype control antibody). The bound proteins were subjected to Western blotting using rabbit anti-TBK1 (catalog no. 3504S; Cell Signaling Technology) and in-house mouse anti-pA137R PAbs.

Sun M., Yu S., Ge H., Wang T., Li Y., Zhou P., Pan L., Han Y., Yang Y., Sun Y., Li S., Li L.F, & Qiu H.J. (2022). The A137R Protein of African Swine Fever Virus Inhibits Type I Interferon Production via the Autophagy-Mediated Lysosomal Degradation of TBK1. Journal of Virology, 96(9), e01957-21.

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ChIP Analysis of BAT Cells

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Chromatin immunoprecipitation (ChIP) assays were performed with 100 mg of cell chromatin extracts from 20 × 10⁶ BAT immortalized cells. DNA was obtained with the Active Motif (Carlsbad, CA) chromatin shearing kit. Chromatin was precipitated by incubation with ChREBP antibody (Novus Biologicals) and thyroid hormone receptor antibody ab2743 (Abcam) at the recommended dilutions at the recommended concentrations or a 1:10,000 dilution of rabbit immunoglobulin G (Abcam) followed by separation with protein G magnetic beads (Active Motif). Binding was analyzed by real-time PCR. Primer sequences are available upon request.

Katz L.S., Xu S., Ge K., Scott D.K, & Gershengorn M.C. (2017). T3 and Glucose Coordinately Stimulate ChREBP-Mediated Ucp1 Expression in Brown Adipocytes From Male Mice. Endocrinology, 159(1), 557-569.

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