Trisodium citrate dihydrate

Gold (III) chloride trihydrate (HAuCl₄. 3H₂O, ≥99.9), fipronil (C₁₂H₄Cl₂F₆N₄OS), (3-aminopropyl)triethoxysilane (APTES; C₉H₂₃NO₃Si, ≥98.0%), sodium chloride (NaCl), tetracycline (C₂₂H₂₄N₂O₈), glycine (C₂H₅NO₂), polyethylene glycol (PEG; C_2nH_4n+2O_n+1), sodium iodide (NaI), and bovine serum albumin (BSA) were all purchased from the renowned company Sigma-Aldrich (St. Louis, MO, USA). Trisodium citrate dihydrate (C₆H₉Na₃O₉) was provided by Kanto Chemical Co., Inc. (Tokyo, Japan). Methyl alcohol (CH₃OH, 99.5%) was purchased from Samchun Pure Chemical Co., Ltd. (Pyeongtaek-si, South Korea). LB broth was purchased from BD DIFCO. The maleic anhydride-activated plate (8-well strip) and phosphate-buffered saline (PBS; C_l2H₃K₂Na₃O₈P₂) were provided by Thermo Fisher (Waltham, MA, USA). The phage display library kit and Escherichia coli ER2738 strain were procured from New England Biolabs (Ipswich, MA, USA). The glass substrate was purchased from the Korea Testing & Research Institute (Gwacheon, Korea). The absorbance spectra were recorded on a V-770 UV–Visible/NIR Spectrophotometer (South Korea). The characterizations of the synthesized gold nanoparticles (AuNPs) were analyzed using field-emission scanning electron microscopy (FE–SEM; JITACHI S-4300 system) and field-emission transmission electron microscopy (FE-TEM; JEM2100F, JEOL Ltd., Peabody, MA, USA).

Methacrylic acid (MAA), N,N′-methylenebisacrylamide (MBAA), ethylene glycol dimethacrylate (EDMA), phenobarbital sodium salt, caffeine anhydrous, theobromine, and THO were all purchased from Wako Pure Chemical Industry (Osaka, Japan). N,N-dimethylformamide (DMF), and trisodium citrate dihydrate were purchased from Kanto Chemical Co., Ltd. (Tokyo, Japan). Both MAA and EDMA were distilled under reduced pressure in the presence of a hydroquinone inhibitor. The distilled monomers were stored in the dark at room temperature and in a refrigerator, respectively, before use. Bovine blood for testing was purchased from the Tokyo Shibaura Zoki Corporation (Tokyo, Japan) (5 g/L trisodium citrate dihydrate was added into the blood as an anticoagulant). Spherical graphite particles of diameter 8 µm (SG-BH8), donated by Ito Graphite Co., Ltd. (Kuwana, Japan), were used as the MIP substrate. The conductive ink (LC3111) for the paper was obtained from ePRONICS Co. Ltd. (Tokyo, Japan). Inkjet photo paper (265 µm in thickness and A4 size) was locally purchased from Sanwa Supply Co., Ltd. (Okayama, Japan). Thermally adhesive PET films LZ-A4100 (100 μm) were obtained from Iris Ohyama, Inc. (Sendai, Japan). Silver/silver chloride (Ag/AgCl) ink was purchased from ALS Co., Ltd. (Tokyo).

Deionized water (resistivity: 18.2 MΩ cm⁻¹), which was obtained from a Milli-Q water purification system, was utilized for the preparation of all aqueous solutions. Hydrogen tetrachloroaurate(III) tetrahydrate (HAuCl₄·4H₂O, Nacalai Tesque), trisodium citrate dihydrate (Kanto Chemical, Japan), L-ascorbic acid (Fujifilm Wako Pure Chemical, Japan), palladium(II) chloride (PdCl₂, Fujifilm Wako Pure Chemical, Japan), hydrochloric acid (HCl, Fujifilm Wako Pure Chemical, Japan), hydroxypropyl cellulose (Sigma-Aldrich, USA), 2-propanol (Kishida Chemical, Japan), ammonium aqueous solution (28 wt%, Kishida Chemical, Japan), titanium diisopropoxide bis(acetylacetonate) (TDAA, Sigma-Aldrich, USA), and 8-ArmPEG-Amine (20,000 Da, Biopharma PEG Scientific, USA) were used as received. Dulbecco's Modified Eagle's Medium (D-MEM, low glucose) with L-glutamine and phenol red and PBS(-) were obtained from Fujifilm Wako Pure Chemical, Japan. Penicillin streptomycin, trypsin–EDTA (0.25%), and fetal bovine serum (FBS) were obtained from Gibco, USA. HeLa cell lines were purchased from JCRB Cell Bank, Japan.

Enrofloxacin, ciprofloxacin, ofloxacin, norfloxacin, tetracycline, creatine monohydrate, bovine serum albumin (BSA), polyvinyl alcohol (PVA), Tween 20, and Gold (III) chloride trihydrate (HAuCl4.3H2O, ≥99.9) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Ampicillin sodium and chloramphenicol were purchased from Duchefa Biochemie (Haarlem, Netherlands). Trisodium citrate dihydrate was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Anti-Enrofloxacin monoclonal antibody (ENR-Ab) produced in mice and coating antigen BSA-ENR were purchased from Creative Diagnostics, (New York, USA). The backing card, absorbent pad, sample pad, nitrocellulose membrane, and conjugation pad used to fabricate LFIA strips, were purchased from Bore Da Biotech Co., Ltd. (Seongnam, Korea). Milk samples were purchased from a local market in Incheon, South Korea. All other reagents and solvents used were of analytical grade or higher.