Cd4 fitc rm4 5

Manufactured by BD

The CD4 FITC (RM4-5) is a fluorescently labeled antibody that binds to the CD4 surface protein. It is used for the identification and quantification of CD4+ T cells in flow cytometry applications.

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Lab products found in correlation

Fc block anti cd16 32, by BD (2 mentions) Armenian hamster anti mouse igg for cd80 percp cy5.5 16 10a1, by BD (2 mentions) Cd11c fitc n418, by BioLegend (2 mentions) Cyan adp lx, by Beckman Coulter (1 mentions) Live dead aqua, by Thermo Fisher Scientific (1 mentions) B220 apc cy7 ra3 6b2, by BD (1 mentions) Cd45 apc, by Thermo Fisher Scientific (1 mentions) Cytoflex, by Beckman Coulter (1 mentions)

Close spelling variants of this product

CD4-FITC (228 citations) RM4-5 (42 citations) CD4 (RM4-5, (35 citations) Anti-CD4-FITC (RM4-5, (3 citations) FITC-conjugated anti-CD4 (RM4–5 clone; (3 citations) Anti-CD4-Pacific Blue and anti-CD4-FITC (clone RM4-5, (2 citations) FITC Rat Anti-Mouse CD4 (Clone RM4-5, (2 citations)

3 protocols using cd4 fitc rm4 5

Isolation of Inflammatory Leukocytes from CNS

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Inflammatory leukocytes infiltrating into the CNS were isolated using an established protocol (3,4). In short, CNS tissue was minced and leukocytes were isolated using a two‐step Percoll gradient (90% and 63%). The isolated cells were collected and then washed prior to staining. Cells were incubated in an anti‐CD16/32 Fc Block (BD Biosciences, San Jose, CA) at a dilution of 1:200. Cells were stained with fluorescently tagged rat anti‐mouse IgG for the following cell surface antibodies, Ly6G FITC (1A8), CD11b PE (M1/70), CD45 BV510 (30‐F11), CD8 PE‐Cy7 (53–6.7), (BD Biosciences), Ly6C APC (HK1.4), CD4 BV785 (RM4‐5), F4/80 FITC (BM8), I‐A/I‐E APC (M5/114.15.2), CD11c FITC (N418) (Biolegend, San Diego, CA), and CD4 FITC (RM4‐5) or Armenian hamster anti‐mouse IgG for CD80 PerCP‐Cy5.5 (16‐10A1) (BD Biosciences). A full description of gating strategies for flow cytometric staining and intracellular cytokine staining is provided in the Supporting Information Figs. S1–S4.

Grist J.J., Marro B.S., Skinner D.D., Syage A.R., Worne C., Doty D.J., Fujinami R.S, & Lane T.E. (2018). Induced CNS expression of CXCL1 augments neurologic disease in a murine model of multiple sclerosis via enhanced neutrophil recruitment. European Journal of Immunology, 48(7), 1199-1210.

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Isolation of Inflammatory Leukocytes from CNS

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Inflammatory leukocytes infiltrating into the CNS were isolated using an
established protocol (3,4). In short, CNS tissue was minced and leukocytes were
isolated using a two-step Percoll gradient (90% and 63%). The
isolated cells were collected and then washed prior to staining. Cells were
incubated in an anti-CD16/32 Fc Block (BD Biosciences, San Jose, CA) at a
dilution of 1:200. Cells were stained with fluorescently tagged rat anti-mouse
IgG for the following cell surface antibodies, Ly6G FITC (1A8), CD11b PE
(M1/70), CD45 BV510 (30-F11), CD8 PE-Cy7 (53-6.7), (BD Biosciences), Ly6C APC
(HK1.4), CD4 BV785 (RM4-5), F4/80 FITC (BM8), I-A/I-E APC (M5/114.15.2), CD11c
FITC (N418) (Biolegend, San Diego, CA), and CD4 FITC (RM4-5) or Armenian hamster
anti-mouse IgG for CD80 PerCP-Cy5.5 (16-10A1) (BD Biosciences). A full
description of gating strategies for flow cytometric staining and intracellular
cytokine staining is provided in the Supplemental Figures
1–4.

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Comprehensive Lymph Node Immune Cell Analysis

Cited 1 time

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Single cell suspensions from pooled inguinal lymph nodes of each mouse were treated with erythrocyte lysis buffer (20 mM Tris/HCl, 155 mM NH₄Cl, pH = 7.2) and washed with 5% FBS in PBS. Cell counts were determined using a Neubauer hemocytometer. Cells were stained with fluorescently-labeled mAbs as previously described (27 (link)). The following antibodies were used: B220-APCCy7 (RA3-6B2; BD Biosciences, San Jose, CA), CD4-FITC (RM4-5; BD Biosciences), CD95-Biotin (15A7; eBioscience), Streptavadin-PE (BioLegend, San Diego, CA), GL7-EF450 (GL-7; eBioscience), PD-1-PECy7 (RMP1-30; BioLegend), CXCR5-APC (L138D7; BioLegend), FcεRI-FITC (MAR-1; BioLegend), CD200R-PE (OX-108; BioLegend), cKit-APC (2B8; eBioscience), CD45-APC (104; eBioscience) CD49b-PerCPCy5.5 (DX5; BioLegend), phosphorylated p38-PECy7 (pT180/pY182; BD Biosciences), and CD41-BV421 (WMReg30; BD Biosciences). Cell viability was determined using live/dead Aqua (Invitrogen, Carlsbad, CA) and doublets were excluded based on forward scatter and pulse width. Samples were acquired on a CyAn ADP LX (Beckman Coulter, Brea, CA) or CytoFLEX (Beckman Coulter) and analyzed using FlowJo software version 10.1r7 (Treestar, Ashland, OR). Gates were determined using fluorescence-minus-one staining controls.

Chandrasekhar J.L., Cox K.M., Loo W.M., Qiao H., Tung K.S, & Erickson L.D. (2019). Cutaneous Exposure to Clinically Relevant Lone Star Ticks Promote IgE Production and Hypersensitivity Through CD4+ T Cell- and MyD88-Dependent Pathways in Mice. Journal of immunology (Baltimore, Md. : 1950), 203(4), 813-824.

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