The following strains belonging to American Type Culture Collection (ATCC; LGC Standards S.r.l., Sesto San Giovanni, Milan, Italy) have been taken into account for the determination of antibacterial activity: Staphylococcus aureus ATCC 6538; Enterococcus faecalis VAN B V583 E; Listeria monocytogenes ATCC 7644; Escherichia coli ATCC 15325; Pseudomonas aeruginosa ATCC 27853; Salmonella tiphymurium ATCC 14028.
Bacterial strains stored at −80 °C in glycerol suspension were sowed on plates containing tryptic soy agar (TSA—Thermo Fisher Scientific, Milan, Italy) and incubated overnight at 37 °C. Subsequently, one colony from these cultures was inoculated in a culture broth of brain heart infusion and incubated at 37 °C for 24 h to obtain freshly cultured microbial suspensions.
Bacterial strains stored at −80 °C in glycerol suspension were sowed on plates containing tryptic soy agar (TSA—Thermo Fisher Scientific, Milan, Italy) and incubated overnight at 37 °C. Subsequently, one colony from these cultures was inoculated in a culture broth of brain heart infusion and incubated at 37 °C for 24 h to obtain freshly cultured microbial suspensions.