Finasteride

Methanol and acetonitrile were purchased from J.T. & Baker (Pennsylvania, USA). Hydrochloride was purchased from JUNSEI (Tokyo, Japan). Phosphoric acid and sodium phosphate dibasic anhydrous (Na₂HPO₄) were purchased from SAMCHUN (Sung-nam, Korea). Borate buffer (3-mercaptopropionic acid in Borate buffer) and OPA (Ortho-Phthal aldehyde) was purchased from Agilent (Santa Clara, (California), USA). GABA standard material was purchased from Sigma-Aldrich (St. Louis, (Missouri), USA). Finasteride was purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan).

Testosterone (>98%) and finasteride (>98%) were obtained from Tokyo Chemical Industry (Kita-ku, Tokyo, Japan). Linoleic acid and β-sitosterol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against β-catenin, Lef-1, TGF-β, Cyclin E, Smo, Shh, β-actin and peroxidase-conjugated antigoat were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Cyclin D1, Bcl-2, Bax, and peroxidase-conjugated antirabbit antibodies were obtained from Cell Signaling Technology (Boston, MA, USA). Gli-1 antibody was purchased from Abcam (Cambridge, UK). BICYCLO was provided from the Laboratory of Synthetic Pharmaceutical Chemistry at Inje University (Gimhae, Korea). All other reagents used in this study were of the highest grade commercially available.