Primary antibodies used for western blotting were anti CBP (Anti-Calmodulin Binding Protein; DAM1411288; Millipore) used at 1∶5000, anti HA (Anti influenza hemagglutinin; H3663; Sigma) used at 1∶5000, anti CTD which recognizes Rpb1 and will be referred to in the text as anti Rpb1 (ab5408; Abcam) used at 1∶500, or finally anti PAP (Peroxidase-Anti-Peroxidase; P1291; Sigma) used at 1∶10000 and rabbit polyclonal anti Ccr4 which was generated in our laboratory and used at 1∶5000. The secondary antibodies were anti-Mouse-HRP (IgG-Peroxidase conjugate; A9044; Sigma) used at 1∶10000 or anti-Rabbit-HRP (IgG-Peroxidase conjugate; A8275; Sigma) used at 1∶10000. For detection of Rpb1, ovaries were incubated with 7G5 mouse monoclonal antibody used at 1∶1000 (a kind gift of Dr. László Tora, Strasbourg).
Dam1411288
Manufactured by Merck Group
DAM1411288 is a laboratory equipment designed for specialized tasks. It functions as a core component in various experimental setups. The product's technical specifications and capabilities are detailed in the accompanying documentation from the manufacturer.
Automatically generated - may contain errors
2 protocols using dam1411288
1
Western Blotting Antibody Panel
2
Immunoblotting of Protein Complexes
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The following antibodies were used: Peroxidase-Anti-Peroxidase (P1291; Sigma) to detect the protein A domain of TAP, anti-CBP against the Calmodulin Binding Protein domain of TAP (DAM1411288; Millipore), a monoclonal anti-HA antibody (H3663; Sigma), a monoclonal anti-c-MYC antibody (M5546; Sigma). Polyclonal antibodies against Taf9, Spt3 and Not5 were produced in rabbits (Elevage Scientifique des Dombes, France) and used at a 1:5000 dilution. The secondary antibodies were anti-Mouse-HRP (IgG-Peroxidase conjugate; A9044; Sigma) used at 1: 10 000 or anti-Rabbit-HRP (IgG-Peroxidase conjugate; A8275; Sigma) used at 1:10 000.
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