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Efluor660 conjugated anti eomes wd1928

Manufactured by Thermo Fisher Scientific
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The EFluor660-conjugated anti-EOMES (WD1928) is a flow cytometry reagent used for the detection and analysis of EOMES-expressing cells. It is a monoclonal antibody conjugated to the EFluor660 fluorescent dye, which emits light in the far-red spectrum. This product can be used to identify and characterize EOMES-positive cell populations in various biological samples.

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Spelling variants of this product

Eomes (10 citations) Anti-Eomes (4 citations) Eomes eFluor660 (3 citations) EOMES (WD1928) (2 citations)

2 protocols using efluor660 conjugated anti eomes wd1928

1

Immunophenotyping of Neural Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
We thawed and permeabilized the cells by resuspending and incubating for 10 minutes on ice in 1×PBS, 0.1% Triton X-100 (Sigma), 1%BSA, 0.0025% RNasin Plus. We incubated one million cells in staining buffer (SB) (1×PBS, 1%BSA, 0.0025% RNasin Plus) with primary antibodies for 30 min at 4°C. Antibodies used were: Alexa488 or PE-conjugated anti-PAX6 (O18-1330; BD Biosciences), PE-conjugated anti-DCX (30/Doublecortin; BD Biosciences), PerCP-Cy5.5-conjugated anti-SOX2 (O30-678; BD Biosciences), eFluor660-conjugated anti-EOMES (WD1928; eBioscience), rabbit anti-CYR61 (D4H5D; Cell Signaling Technology) followed by PE-conjugated goat anti-rabbit IgG (Life Technologies). We washed cells in SB, resuspended in SB containing 1 µg/mL DAPI (Life Technologies), and filtered prior to sorting.
Thomsen E.R., Mich J.K., Yao Z., Hodge R.D., Doyle A.M., Jang S., Shehata S.I., Nelson A.M., Shapovalova N.V., Levi B.P, & Ramanathan S. (2015). Fixed single-cell transcriptomic characterization of human radial glial diversity. Nature methods, 13(1), 87-93.
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2

Immunophenotyping of Neural Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
We thawed and permeabilized the cells by resuspending and incubating for 10 minutes on ice in 1×PBS, 0.1% Triton X-100 (Sigma), 1%BSA, 0.0025% RNasin Plus. We incubated one million cells in staining buffer (SB) (1×PBS, 1%BSA, 0.0025% RNasin Plus) with primary antibodies for 30 min at 4°C. Antibodies used were: Alexa488 or PE-conjugated anti-PAX6 (O18-1330; BD Biosciences), PE-conjugated anti-DCX (30/Doublecortin; BD Biosciences), PerCP-Cy5.5-conjugated anti-SOX2 (O30-678; BD Biosciences), eFluor660-conjugated anti-EOMES (WD1928; eBioscience), rabbit anti-CYR61 (D4H5D; Cell Signaling Technology) followed by PE-conjugated goat anti-rabbit IgG (Life Technologies). We washed cells in SB, resuspended in SB containing 1 µg/mL DAPI (Life Technologies), and filtered prior to sorting.
Thomsen E.R., Mich J.K., Yao Z., Hodge R.D., Doyle A.M., Jang S., Shehata S.I., Nelson A.M., Shapovalova N.V., Levi B.P, & Ramanathan S. (2015). Fixed single-cell transcriptomic characterization of human radial glial diversity. Nature methods, 13(1), 87-93.
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