Plastic culture dishes

Manufactured by Corning

Sourced in United States

Corning's plastic culture dishes are sterile, single-use containers designed for cell and tissue culture applications. They provide a flat surface for the growth and maintenance of cells in a controlled environment. These dishes are made of high-quality, durable plastic materials that are compatible with a variety of cell culture media and techniques.

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Lab products found in correlation

Caco 2 cell line, by American Type Culture Collection (3 mentions) 24 well plastic cell culture clusters, by Corning (2 mentions) Penicillin, by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) Minimum essential medium (mem), by Merck Group (2 mentions) Amphotericin b, by Merck Group (1 mentions) Caco 2, by American Type Culture Collection (1 mentions) Caco 2, by Corning (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Caco 2 cells, by Merck Group (1 mentions) Caco 2 cells, by Corning (1 mentions) 24 well plate, by Corning (1 mentions) Antibiotic antimycotic, by Merck Group (1 mentions) Rpmi 1640 medium, by Corning (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Collagenase a, by Merck Group (1 mentions)

9 protocols using plastic culture dishes

Caco-2 Cell Culture Protocol

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The Caco-2 cell line was obtained from American Type Culture Collection (HTB-37; Manassas, VA, USA) and was used between passage numbers 8–18. The cells, maintained in a humidified atmosphere (5% CO₂ and 95% air), were grown in Minimum Essential Medium containing glucose (5.55 mM), fetal calf serum (15%), HEPES (25 mM), amphotericin B (0.25 μg/mL), streptomycin (100 μg/mL), and penicillin (100 units/mL). The culture medium was replaced every 3–4 days, and the culture was split every 10 days. For sub-culturing, the cells were removed enzymatically (0.25% trypsin-EDTA, 5 min, 37 °C), split 1:3, and sub-cultured in plastic culture dishes (21 cm²; ∅ 60 mm; Corning Costar, Corning, NY, USA).
To perform the assays, Caco-2 cells were seeded on 24-well plastic cell culture clusters (2 cm²; ∅ 16 mm; Corning Costar) and were used 10 days after the initial seeding (100% confluence, corresponding to an average number of 2 × 10⁵ cells). The culture medium was made free of fetal bovine serum for 24 h before the experiments.

Peixoto J.A., Andrade N., Machado S., Costa A.S., Oliveira M.B., Martel F, & Alves R.C. (2022). Green/Roasted Coffee and Silverskin Extracts Inhibit Sugar Absorption by Human Intestinal Epithelial (Caco-2) Cells by Decreasing GLUT2 Gene Expression. Foods, 11(23), 3902.

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Cell Culture Conditions for IEC-6 and Caco-2 Lines

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The IEC-6 and Caco-2 cell lines were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Braunschweig, Germany) and used between passages numbers 19-27 (IEC-6 cells) and 23-40 (Caco-2 cells). The cells were maintained in a humidified atmosphere of 5% CO₂ /95% air. IEC-6 cells were cultured in Dulbecco’s Modified Eagle’s Medium: RPMI 1640 medium (1:1), supplemented with 10% fetal bovine serum, 0.1 U/ml insulin, 5.96 g HEPES, 2.2 g NaHCO₃ , 100 U/ml penicillin, 100 mg/ml streptomycin and 0.25 mg/ml amphotericin B (all from Sigma, St. Louis, MI, USA). Caco-2 cells were cultured in Minimum Essential Medium containing 5.55 mM glucose, 15% fetal calf serum, 25 mM HEPES, 100 U/ml penicillin, 100 mg/ml streptomycin and 0.25 mg/ml amphotericin B (all from Sigma, St. Louis, MI, USA). Culture medium was changed every 2-3 days and the culture split every 7 days. For subculturing, the cells were treated with 0.25% trypsin-EDTA (5 minutes, 37˚C), split 1:3, and subcultured in plastic culture dishes (21-cm², Corning Costar, Corning, NY, USA). For the experiments, cells were seeded on 24-well plastic cell culture clusters (1.9 cm², TPPs, Trasadingen, Switzerland), and the experiments were performed 8-10 days after the initial seeding.

Couto M.R., Gonçalves P., Catarino T.A, & Martel F. (2017). The Effect of Inflammatory Status on Butyrate and Folate Uptake by Tumoral (Caco-2) and Non-Tumoral (IEC-6) Intestinal Epithelial Cells. Cell Journal (Yakhteh), 19(Suppl 1), 96-105.

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Caco-2 Cell Culture Protocol

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The Caco-2 (a human colorectal adenocarcinoma cell line) was obtained from ATCC (Manassas, VA, USA) and was used between passage number 40–68. The cells were maintained in a humidified atmosphere of 5% CO₂–95% air and were grown in a Minimum Essential Medium containing 5.55 mM glucose and supplemented with 15% fetal bovine serum (FBS), 25 mM HEPES and 100 units mL⁻¹ penicillin, 100 μg mL⁻¹ streptomycin (all from Sigma-Aldrich, St. Louis, MO, USA). The culture medium was renewed every 3–4 days and the culture was split every 12 days. For sub-culturing, the cells were removed enzymatically (0.25% trypsin–EDTA, 5 min, 37 °C), split 1:3, and subcultured in plastic culture dishes (21 cm²; ∅ 60 mm; Corning Costar, Corning, NY, USA).

Barreto-Peixoto J.A., Silva C., Costa A.S., Álvarez-Rivera G., Cifuentes A., Ibáñez E., Oliveira M.B., Alves R.C., Martel F, & Andrade N. (2023). A Prunus avium L. Infusion Inhibits Sugar Uptake and Counteracts Oxidative Stress-Induced Stimulation of Glucose Uptake by Intestinal Epithelial (Caco-2) Cells. Antioxidants, 13(1), 59.

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Caco-2 Cell Culture Protocol

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The Caco-2 cell line was obtained from ATCC (Manassas, VA, USA) and was used between passage numbers 8 and 18. The cells were grown in Minimum Essential Medium (Sigma, St. Louis, MO, USA) containing 5.55 mM glucose and supplemented with 15% fetal calf serum, 25 mM HEPES, 100 units/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL amphotericin B, in a humidified atmosphere (5% CO₂/95% air). Cells were cultured in plastic culture dishes (21 cm²; ∅ 60 mm; Corning Costar, Corning, NY, USA) with culture medium change every 3–4 days and culture split 1:3 (0.25% trypsin-EDTA, 5 min, 37 °C) every 10 days.
For the experiments, Caco-2 cells were seeded on 24-well plastic cell culture clusters (2 cm²; ∅ 16 mm; Corning Costar) and used at 100% confluence (10 days after the initial seeding). Then, 24 h before the experiments, the culture medium was made free of fetal bovine serum.

Peixoto J.A., Andrade N., Machado S., Costa A.S., Puga H., Oliveira M.B., Martel F, & Alves R.C. (2022). Valorizing Coffee Silverskin Based on Its Phytochemicals and Antidiabetic Potential: From Lab to a Pilot Scale. Foods, 11(12), 1671.

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HT-29 Cell Culture Protocol

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Reagents. RPMI-1640 medium was purchased from Invitrogen (Invitrogen Life Technologies, Paisley, Uk). The following reagents were purchased from Sigma (St. Louis, MO, USA): AD (Adrenaline-L-adrenaline(+)-bitartrate salt), NA (Noradrenaline-L-(-)-noradrenaline(+)-bitartrate salt monohydrate), ISO (Iso prenaline-(-)-isoprenaline(+)-bitartrate salt), PRO (Pro pra nolol-DL-propranolol hydrochloride), ICI- HT-29 cells were cultured in RPMI-1640 medium supplemented with 10% of FbS, 100 U/ml penicillin and 100 µg streptomycin. The cells were grown at 37˚C in a humidified 5% CO 2 atmosphere. Culture medium was changed every 2-3 days. When the cells reached 90-100% confluency, the medium was removed, and the cell monolayer was washed once with PbS. The cell monolayer was treated with 1 ml of 0.25% (w/v) trypsin-EDTA and incubated for 2 min to ensure complete cell detachment. For sub-culturing, the cells were sub-cultured in plastic culture dishes (21 cm 2 , 60-mm diameter, Corning Costar, Corning, NY, USA). For the experiments, HT-29 cells were seeded in 96-well (0.37 cm 2 , 6.9 mm diameter, TPP) or 24-well plastic cell culture clusters (2 cm 2 , 16-mm diameter, TPP) depending on experimental conditions. Experiments were performed 4-5 days after the initial seeding (90-100% confluency).

Coelho M., Moz M., Correia G., Teixeira A., Medeiros R, & Ribeiro L. (2015). Antiproliferative effects of β-blockers on human colorectal cancer cells. Oncology reports, 33(5).

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Caco-2 Cell Culture for Cytotoxicity Assays

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The Caco-2 cell line was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) and was used between passage numbers 56-62. Caco-2 cells (ATCC 37-HTB) were incubated at 37 °C in a humidified atmosphere (95% air; 5% CO 2 ) and were grown in Minimum Essential Medium (Sigma, St. Louis, MO, USA) supplemented with 15% fetal bovine serum, 25 mM HEPES, 100 units/ml penicillin, 100 μg ml -1 streptomycin and 0.25 μg ml -1 amphotericin B. Culture medium was changed every 2-3 days and the culture was split every 7 days. For subculturing, the cells were removed enzymatically (0.25% trypsin-EDTA, 5 min, 37 °C), split 1:3, and subcultured in plastic culture dishes (21 cm 2 ; ∅ 60 mm; Corning Costar, Corning, NY). For the experiments, the Caco-2 cells were seeded on 24-well plates (2 cm 2 ; ∅ 16 mm; Corning Costar). For 24 h before the experiment, the cell medium was free of fetal bovine serum. Uptake and enzymatic studies were generally performed 14 days after the cells formed a monolayer.
Caco-2 cells were treated for 24 h with CPP2 and all conjugates (1-250 μM) except for BTZ-C2-CPP2 (1-100 μM). At the end of the treatment period, proliferation, viability and assessment of cell mass studies were performed. Control cells were exposed to the respective solvent (water).

Duarte D., Fraga A.G., Pedrosa J., Martel F, & Vale N. (2019). Increasing the potential of cell-penetrating peptides for cancer therapy using a new pentagonal scaffold. European journal of pharmacology, 860.

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Isolation of Rat Lung and Human Mesothelial Cells

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Rat lung primary cells were prepared from the lung of a young adult (<1 year-old) Sprague Dawley rat from the Office of Laboratory Animal Care (University of California at Berkeley) following published procedures [14 (link)]. The neoplastic F10 rat clone was derived from a culture of SV40 virus-infected rat lung cells as described by us previously [14 (link)]. Primary human mesothelial cells were prepared and infected with SV40 virus as described by Bocchetta et al. [98 (link)]. The cells were grown on plastic culture dishes (Corning Falcon, NY, USA) in RPMI 1640 medium (Corning Cellgro, MA, USA) supplemented with 3–5% fetal calf serum, 3–5% calf serum (Hyclone, UT, USA), 1% Antibiotic Antimycotic and 1% Nyastin (Sigma Co., St Louis, MI, USA) following published procedures [14 (link),71 (link)].

Hirpara A., Bloomfield M, & Duesberg P. (2018). Speciation Theory of Carcinogenesis Explains Karyotypic Individuality and Long Latencies of Cancers. Genes, 9(8), 402.

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Isolation and Cryopreservation of Porcine Fetal Fibroblasts

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PFFs were derived from whole conceptuses of Large White pigs at day 30 of pregnancy [15 (link)]. Briefly, the heads and internal organs were removed from the conceptuses and washed three times with Ca²⁺ and Mg²⁺ free phosphate-buffered saline supplemented with 1% penicillin and streptomycin. Then, the remnants were minced and digested with 0.25% trypsin-ethylenediaminetetraacetic acid. Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) was added to the medium to inactivate the enzyme. The suspended cells were centrifugated (1,000×g) for 5 min and seeded into plastic culture dishes (Corning, New York, USA). The cells were cultured in DMEM supplemented with 10% FBS, 1 mM sodium pyruvate, 1% nonessential amino acids, and 10 mg/mL penicillin–streptomycin in a humidified atmosphere of 5% CO₂ at 39°C. Unattached cells were removed and attached cells were further cultured until confluency. PFFs were stored in liquid nitrogen with a freezing medium, which containing 80% DMEM, 10% dimethyl sulfoxide (Sigma, St. Louis, MO, USA), and 10% FBS.

Geng H., Hao L., Cheng Y., Wang C., Wei W., Yang R., Li H., Zhang Y, & Liu S. (2019). miR-140 inhibits porcine fetal fibroblasts proliferation by directly targeting type 1 insulin-like growth factor receptor and indirectly inhibiting type 1 insulin-like growth factor receptor expression via SRY-box 4. Asian-Australasian Journal of Animal Sciences, 33(10), 1674-1682.

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Isolation and Expansion of Hemangioma-derived MSCs

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Fresh hemangioma samples were obtained from Jinling Hospital and Children's Hospital in Nanjing, China, in accordance with the human subject's protocol approved by the ethics committee of Jingling Hospital (2012NZGKJ-117). Informed consent was achieved according to the Declaration of Helsinki. MSCs were separated according to the procedure described previously [6 (link), 8 (link)]. In brief, fresh samples were rinsed in chlorhexidine solution and phosphate-buffered saline, minced, digested with 0.2% collagenase A (M9195, Sigma-Aldrich) at 37°C for one and a half hoursand then filtered through 70-μm-cell strainers (BD Falcon^TM) to get single-cell suspension. Afterwards these cells were resuspended by Dulbecco's Modified Eagle's Medium-low glucose (DMEM-LG) (Hyclone)/10%FBS (Hyclone) supplemented with 1×PG (100U/ml penicillin, 100μg/ml gentamycin) (briefly called DMEM-LG in the passage below) and plated on plastic culture dishes (Corning). On the second day, the medium was discarded to remove the floating cells. Cells left were cultured to confluence and then divided into two groups at the ratio of 1:3.

Yuan S.M., Guo Y., Wang Q., Xu Y., Wang M., Chen H.N, & Shen W.M. (2017). Over-expression of PPAR-γ2 gene enhances the adipogenic differentiation of hemangioma-derived mesenchymal stem cells in vitro and in vivo. Oncotarget, 8(70), 115817-115828.

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