6890n chromatograph

The analysis of Artemisia dracunculus essential oil by gas chromatography was carried out using an Agilent 6890N Chromatograph equipped with a flame ionization detector (FID) and an Agilent 5975 inert XL mass detector (MSD) with an ionization energy of 70 eV. Two Teknokroma TR-520232 capillary columns were used. The analysis conditions were: an initial temperature of 40 °C for 14 min; a gradient of 1.5 °C/min up to 250 °C; and an isotherm for 10 min. The injector (and transfer line) temperature was 270 °C. Helium was used as a carrier gas for the 2 L of injected Artemisia dracunculus essential oil in splitless mode at a flow rate of 0.5 mL/min. GC-MS analysis was performed by ESI in a mass range of 24 to 400 m/z with a delay time of 2 min. Identification of Artemisia dracunculus components was performed by comparing the mass spectra to those in the available NIST database (GC-MS) and by their relative retention index (Kovacs Index, KI) towards a mixture of alkanes C₄ to C₂₆, injected in the same experimental conditions (GC-FID).

The catalytic tests were carried out in a tubular reactor with porous plate (ID = 1 cm; L = 35 cm) and prior to the catalytic tests, the samples underwent an activation process at 300 and 500 °C under H₂ flow (1 mg = 1 mL min⁻¹). Flow mass controllers were used to set the flow of a gas mixture at 5000 ppm of C₃H₈ (purity of 99.998%) and 2% of O₂ in N₂ (purity of 99.997%) with a total flow rate of 100 mL min⁻¹. The catalyzed reaction was performed using 40 or 80 mg of Ru/TiO₂ catalyst, from room temperature to 500 °C, with a heating ramp of 3 °C min⁻¹. The effluents were analyzed by gas chromatography in an in-situ Research Rig-150 equipment; the effluent analysis was performed using an Agilent Technologies 6890N chromatograph with CO, CO₂, and C₃H₈ detection. Propane conversion (%) = ([C₃H₈]_in − [C₃H₈]_out/[C₃H₈]_in) × 100.

JALEx was analyzed by gas chromatography combined with mass spectrometry (GC-MS) using an Agilent 6890N chromatograph (Palo Alto, CA) equipped with a capillary column DB-H17HT (30 m × 0.25 mm × 0.15 μm film thickness). Helium was the carrier gas at 1.0 mL/min and inlet pressure of 3.14 psi, 10:1 split/splitless ratio. The oven temperature was programmed from 150°C to 340°C at 4°C/min for 17 minutes. The injection volume was 1 μL of a 2 mg/mL chloroform solution. Data were processed using MSD Productivity Chem Station Software operating with ion source at 250°C and electron impact ionization at 70 eV. Individual components were characterized by their fragmentation patterns in the mass spectrum.

The extracts were analysed using an Agilent 6890 N chromatograph fitted with a 30 m × 0.25 mm (i.d.) fused silica DB-1 capillary column coated with 0.25 μm cross linked polydimethylsiloxane stationary phase. Ultra-high purity nitrogen (1.2 ml/min) was used as the carrier gas. Injector port, column oven, and detector temperatures were 265 °C, 240 °C, and 300 °C, respectively. Flame ionization (FID) was used for analyte detection. Two injections were used for each sample and the results averaged. Permethrin concentration was calculated by comparing permethrin/triphenyl phosphate peak area ratios against a calibration curve generated from solutions containing known permethrin /triphenyl phosphate mass ratios.

Fungal morphological characters were measured using a light microscope at 1000× and photomicrographs were captured with a Nikon DS-L3 camera adapted to a Nikon 80i microscope (Nikon Corp. Mitsubishi, JP). PCR products were purified and sequenced by Macrogen (Seoul, Korea). DNA was amplified using a GeneAmp 9700 DNA Thermal Cycler (PerkinElmer, Life Technology, Carlsbad, CA, USA).
The IR spectrum was recorded with a Nicolet 8700 FTIR spectrometer (Thermo Electron, Madison, WI, USA). Gas chromatography–mass spectrometry (GC-MS) analyses were done with an Agilent Technologies (Santa Clara, CA, USA) 6890N Chromatograph coupled to an Agilent 5975B mass-selective detector as previously described [5 ]. Low- and high-resolution MS spectra were recorded on a GC Mate II mass spectrometer in FAB [+] mode using NBA matrix and JEOL Calibration Ultramark and Resolution 3000 (JEOL, Peabody, MA, USA). ¹H and ¹³C NMR spectra were recorded in an AMX-400 spectrometer (Bruker Corp., Billerica, MS, USA) at 400 and 100 MHz, respectively, using TMS as an internal standard, and in a Varian, Agilent AR Premium Compact (Varian, Palo Alto, CA, USA) at 599.774 and 150.826 MHz, respectively. Chemical shifts were reported in ppm and coupling constants (J) were given in Hz. Two-dimensional experiments (COSY, DEPT, HSQC, and HMBC) were done using the Varian equipment.