Cd11b pe dazzle594 clone m1 70

Manufactured by BioLegend

CD11b-PE/Dazzle594 (clone M1/70) is a fluorochrome-conjugated monoclonal antibody that binds to the CD11b surface antigen. CD11b is an integrin alpha subunit that is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. This product can be used in flow cytometry applications to identify and characterize these cell populations.

Automatically generated - may contain errors

Lab products found in correlation

Cd3 fitc clone17a2, by BioLegend (2 mentions) Facsaria 2 system, by BD (2 mentions) Cd8 percp cy5 5 clone 53 6, by Thermo Fisher Scientific (2 mentions) Cd45 bv510 clone 30 f11, by BioLegend (2 mentions) Cd11b apc clone m1 70, by BioLegend (1 mentions) Ter119 pe cy7, by BioLegend (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) αcd16 32 antibody, by BioLegend (1 mentions) Myelin removal beads 2, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

CD11b (457 citations) CD11b (M1/70, (86 citations) CD11b-PE (81 citations) CD11b (clone M1/70, (72 citations) M1/70 (46 citations) Clone M1/70 (26 citations) CD11b PE (M1/70, (7 citations) CD11b PE (clone M1/70, (6 citations)

3 protocols using cd11b pe dazzle594 clone m1 70

Multiparametric Sorting of NK Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions were stained for antibodies raised against the following antigens: CD45 FITC (clone 30-F11, BioLegend), CD11b PE-Dazzle594 (clone M1/70, BioLegend), TCRβ BUV737 (clone H57-597, BioLegend), NKp46 BV650 (clone 29A1.4, BioLegend), and CD49a BUV395 (clone RM4-5, BD Biosciences), in addition to Live/Dead Near-IR viability stain (#L10119, Thermo Fisher Scientific). NK cells (Live CD45⁺ CD11b^-/low, TCRβ^- CD3^- NKp46⁺) were sorted into CD11b⁺ CD49a^- and CD11b^- CD49a⁺ cells using a FACS Aria II Cell sorter (BD Biosciences) and then used for in vitro assays. For scRNA-seq, singles cells were stained using antibodies against the following: CD45-BUV395 (clone 30-F11, BioLegend), CD11b-APC (clone M1/70, BioLegend), Ter119-PE-Cy7 (1:400, clone TER-119, BioLegend), NK1.1-BV650 (clone PK136, BioLegend). Tumor infiltrating lymphocytes (Live CD45⁺ Ter119^- CD11b^-/low) were sorted into two groups based on the presence of the Kaede Red, namely, G48 and R48 referring to Kaede Green 48 h and Kaede Red 48 h.

Dean I., Lee C.Y., Tuong Z.K., Li Z., Tibbitt C.A., Willis C., Gaspal F., Kennedy B.C., Matei-Rascu V., Fiancette R., Nordenvall C., Lindforss U., Baker S.M., Stockmann C., Sexl V., Hammond S.A., Dovedi S.J., Mjösberg J., Hepworth M.R., Carlesso G., Clatworthy M.R, & Withers D.R. (2024). Rapid functional impairment of natural killer cells following tumor entry limits anti-tumor immunity. Nature Communications, 15, 683.

+ Open protocol

+ Expand

Multiparameter Analysis of Tumor-Infiltrating Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated TIL from GL261-OVA tumors were blocked with αCD16/32 antibody (Biolegend) and stained with PE/APC H-2K^b-SIINFEKL dextramer (Immudex) according to the manufacturer’s staining protocol. TIL were subsequently stained with fixable viability dye eFluor 780 (1:1000, Thermo Fisher, 65-0865-14), CD45-BV510 (clone 30-F11, Biolegend), CD11b-PE/Dazzle594 (clone M1/70, Biolegend), CD3-FITC (clone 17A2, Biolegend), and CD8-PercpCy5.5 (clone 53-6.7, Thermo Fischer). TIL were analyzed on a FACSAria II system (BD Bioscience).

Blobner J., Kilian M., Tan C.L., Aslan K., Sanghvi K., Meyer J., Fischer M., Jähne K., Breckwoldt M.O., Sahm F., von Deimling A., Bendszus M., Wick W., Platten M., Green E, & Bunse L. (2021). Comparative evaluation of T-cell receptors in experimental glioma-draining lymph nodes. Neuro-oncology Advances, 3(1), vdab147.

+ Open protocol

+ Expand

Purification of Tumor-Infiltrating CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For purification of GL261 TILs, myelin removal was performed. Myelin removal beads II (Miltenyi Biotec; 130-096) were added to a tumor single-cell suspension and processed according the manufacturer’s protocol. Cells from splenocytes, draining cervical lymph nodes, inguinal lymph nodes, and GL261 infiltrating TIL were stained with Propidium iodide (PI) (Sigma-Aldrich; P4864) for exclusion of dead cells, CD45-BV510 (clone 30-F11, Biolegend), CD11b-PE/Dazzle594 (clone M1/70, Biolegend), CD3-FITC (clone 17A2, Biolegend), and CD8-PercpCy5.5 (clone 53-6.7, Thermo Fischer). CD8⁺ T cells were isolated by sorting for PI^-CD45⁺CD3⁺CD8⁺ cells on the FACSAria II system (BD Biosciences) using FACSDiva software.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!