Single-cell suspensions from BM and spleen were treated with Gey’s solution to remove red blood cells and resuspended in PBS supplemented with 2% BSA. The cells were then stained with a combination of fluorescence-conjugated antibodies. APC-conjugated anti-B220, anti-IgM, anti-CD4 and anti-CD44, and PE-Cy7-conjugated anti-B220, anti-IFNγ, anti-CD25 and anti-CD23 were purchased from eBioscience. PE-conjugated anti-CD43, anti-CD21, anti-Thy1.2, anti-CD5, anti-CD8, and anti-IgD were purchased from BD Biosciences. Samples were applied to a flow cytometer (LSRII, Becton Dickinson). Data were collected and analyzed using FACSDiva software (Becton Dickinson) or FlowJo software (Tree Star).
Apc conjugated anti b220
Manufactured by Thermo Fisher Scientific
Sourced in United States, Germany
The APC-conjugated anti-B220 is a fluorescent-labeled antibody product designed for the detection and analysis of B220-positive cells. It is a tool for immunophenotyping and cell sorting applications in flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Anti ifn γ, by Thermo Fisher Scientific (1 mentions)
Anti cd8, by BD (1 mentions)
Anti igd, by BD (1 mentions)
Anti cd21, by BD (1 mentions)
Anti cd5, by BD (1 mentions)
Anti thy1, by BD (1 mentions)
Anti cd23, by Thermo Fisher Scientific (1 mentions)
Pe cy7 conjugated anti b220, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti cd43, by BD (1 mentions)
Facsdiva software, by BD (1 mentions)
Confocal microscope lsm700, by Zeiss (1 mentions)
Pe conjugated anti cd4, by BioLegend (1 mentions)
Percp conjugated anti cd8, by BD (1 mentions)
Fitc conjugated anti f4 80, by Thermo Fisher Scientific (1 mentions)
Allophycocyanin apc conjugated anti cd11b, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti cd11c, by Thermo Fisher Scientific (1 mentions)
Apc conjugated anti epcam, by Thermo Fisher Scientific (1 mentions)
Rapamycin, by Merck Group (1 mentions)
3 protocols using apc conjugated anti b220
1
Multiparametric Flow Cytometry Analysis
2
Multimodal Immune Profiling of Tissue
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Th17 (CD4, IL-17), Treg (CD4, CD25, Foxp3), SMILE, AMPK, mTOR, pSTAT3 705, pSTAT3 727 and germinal center markers (CD3, B220, PNA) expression levels were analyzed by confocal microscopy. Tissue Sects. (7 μm thick) were fixed in methanol-acetone and stained with phycoerythrin (PE)-conjugated anti-CD4 (Biolegend, San Diego, CA, USA), fluorescein isothiocyanate (FITC)-conjugated anti CD4 (BD Bioscience, San Diego, CA, USA), PE-conjugated anti-interleukin-17 (eBioscience, San Diego, CA, USA), allophycocyanin (APC)-conjugated anti-CD25 (Biolegend), PE-conjugated anti-Foxp3 (Thermo Fisher Scientific, Waltham, MA, USA), PE-conjugated anti-phosphorylated Stat3 (Tyr 705, Ser 727, BD Bioscience), APC-conjugated anti-B220 (eBioscience), Alexa 594-conjugated anti-PNA (Thermo Fisher Scientific), SMILE (Abcam, Cambridge, UK), AMPK (Abcam) and mTOR (Cell Signaling). After overnight incubation at 4℃, the stained sections were analyzed on a Zeiss confocal microscope (LSM 700; Carl Zeiss, Oberkochen, Germany) at 200× magnification and Zen Blue edition software.
3
Psoriasis Induction and Analysis Protocol
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Aldara ® (IMQ) 5% cream (MEDA Pharmaceuticals, Vienna, Austria) was used for inducing psoriatic skin changes in the mice. 1% rapamycin (Merck Calbiochem, Darmstadt, Germany) ointment was made by mixing 50 mg petroleum jelly with 10 μl of the corresponding rapamycin stock, 50 mg/ml. P-mTOR S2448 #2976 and P-S6 S235/6 #2211 antibodies were from Cell Signaling Technology, Frankfurt, Germany. Keratin 6 antibody (Ks6. KA12) from Thermo Scientific, Darmstadt, Germany and caspase-14 antibody (NB100-56126) was from Novus Biologicals, Wiesbaden, Germany. NIMP-R14 Ly-6G/C (ab2557) was from Abcam, Cambridge, UK. Fluorescein isothiocyanate (FITC)conjugated anti-CD3, phycoerythrin (PE)-conjugated anti-CD4, PE-conjugated anti-CD11c, allophycocyanin (APC)-conjugated anti-CD11b, FITC-conjugated anti-F4/80, APC-conjugated anti-B220, PE-conjugated anti-langerin, APC-conjugated anti-EpCAM all were from eBioscience, Darmstadt, Germany, while peridinin chlorophyll protein complex (PerCP/Cy5)-conjugated anti-Siglec-H was from Biolegend, Koblenz, Germany and PerCP-conjugated anti-CD8 was purchased from BD Bioscience, Heidelberg Germany.
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