Gamma irradiated 10 kda cut off cassettes

Manufactured by Thermo Fisher Scientific

Gamma-irradiated 10 kDa cut-off cassettes are a type of lab equipment designed for molecular weight separation and purification. The cassettes feature a 10 kDa molecular weight cut-off, which allows for the retention of molecules above this threshold while permitting the passage of smaller molecules. These cassettes are gamma-irradiated to ensure sterility.

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Lab products found in correlation

Bca assay, by Thermo Fisher Scientific (2 mentions) Glycolaldehyde dimer, by Merck Group (1 mentions) Bovine serum albumin solution, by Merck Group (1 mentions)

Spelling variants of this product

GAMMA-10 (1 citations)

2 protocols using gamma irradiated 10 kda cut off cassettes

Glycolaldehyde-Derived AGEs Generation

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Glycolaldehyde-derived AGEs were generated under sterile conditions as described by Valencia et al.^{59 (link)}. Briefly, sterile filtered 30% BSA solution (Sigma, St. Louis, MO) was incubated with 70 mM glycolaldehyde dimer (Sigma) in sterile PBS without calcium chloride and magnesium chloride for three days at 37 °C. After incubation, the AGE product was dialyzed against sterile PBS for 24 hours at 4 °C using gamma-irradiated 10 kDa cut-off cassettes (Thermo Scientific, Waltham, MA) to remove unreacted glycolaldehyde. Unmodified control BSA was prepared similarly, without the addition of glycolaldehyde dimer. Protein concentration was determined by BCA assay (Thermo Scientific) and absence of endotoxin (<0.25Eu/ml) was confirmed via the LAL gel-clot assay (GenScript, Piscataway, NJ).

Patel S.H., Yue F., Saw S.K., Foguth R., Cannon J.R., Shannahan J.H., Kuang S., Sabbaghi A, & Carroll C.C. (2019). Advanced Glycation End-Products Suppress Mitochondrial Function and Proliferative Capacity of Achilles Tendon-Derived Fibroblasts. Scientific Reports, 9, 12614.

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Glycolaldehyde-Derived Advanced Glycation Endproducts

Cited 2 times

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Glycolaldehyde-derived advanced glycation end-products (AGEs) were generated under sterile conditions as we [37 (link),38 (link)] and others [46 (link)] have described previously. Briefly, sterile filtered 30% bovine serum albumin (BSA) solution (Sigma, St. Louis, MO) was incubated with 70 mM glycolaldehyde dimer (Sigma) in sterile PBS without calcium chloride and magnesium chloride for three days at 37°C. Next, the AGE product was dialyzed against sterile PBS for 24 hours at 4°C using gamma-irradiated 10kDa cut-off cassettes (Thermo Scientific, Waltham, MA) to remove unreacted glycolaldehyde dimer. Unmodified control BSA was prepared similarly, without the addition of glycolaldehyde dimer. Protein concentration was determined by BCA assay (Thermo Scientific), and the extent of BSA modification was confirmed by fluorescence, absorbance, and loss of primary amines [46 (link)–49 (link)].

Patel S.H, & Carroll C.C. (2022). IMPACT OF ELEVATED SERUM ADVANCED GLYCATION END PRODUCTS AND EXERCISE ON INTACT AND INJURED MURINE TENDONS. Connective tissue research, 64(2), 161-174.

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