Bamh 1 and not 1

The mitochondrial location vector pDsRED2-Mito (pDRM) was purchased from Waryong Biotechnology (Beijing, China). Homologous recombination was performed to clone PCK2 coding sequences into pDRM, according to the instructions of the one-step cloning kit ClonExpress II (Vazyme, Nanjing, China). Briefly, pDRM was linearized by restriction enzyme BamH I and Not I (NEB, Ipswich, MA, USA). PCK2 mRNA sequence was cloned using primers containing BamH I and Not I restriction sites and homologous sequences with the terminal sequences of linearized vector at the 5′-end (Suppl. Table S1). Then the linearized vector and the PCK2 coding sequences were ligated using ligase Exnase II supplied by the kit, and the ligation product was transfected into DH.5α. The monoclonal was picked, and pDRM-PCK2 vectors were purified using GeneJET Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA, USA) and verified by sequencing.

A549 cell line, H7N9 and H1N1pdm09 influenza virus were obtained from Shenzhen Center for Disease Control and Prevention (SZCDC). All the reagents for DIGE were purchased from GE Healthcare (Pittsburgh, PA, USA). DL-Dithiothreitol (DTT), Iodoacetamide (IAA), CHAPS were purchased from Sigma–Aldrich (Louis, MO, USA). The antibodies against CAPZA1, OAT, PCBP1, EIF5A, PAFAH1B2 were purchased from Abcam (Cambridge, MA, USA). The antibody against β-action was purchased from Santa Cruz (Santa Cruz, CA, USA). The secondary antibodies of goat anti-mouse and goat anti-rabbit were purchased from Thermo Fisher (Rockford, IL, USA). The restriction enzyme (BamH I and Not I) were purchased from NEB(USA).
Lipofectamine2000 and pcDNA3.1/Neo(+) were purchased from Invitrogen (Carlsbad, CA, USA).