The minerals formed in pure bacterial cultures were extracted from the solid media. The bacterial biomass was stripped from the surface of the solid medium by slightly scraping the top layer with a sterile scalpel. The mineral crystals were washed three times with 15 mL distilled water. This process does not alter the shape of the crystals, as confirmed by optical microscopy before and after recovery [10] . The overnight dried samples at 37 °C, were analyzed by SEM/EDS and XRD techniques.
The SEM analysis was conducted utilizing a Nova Nano Scanning Electron Microscopy equipped with a Bruker EDX Detector with a magnification of 200,000X and a resolution of 5 nm. The EDS was obtained in accordance with the “ASTM standard method E1508–12a”, using a spot size of 5 and an accelerating voltage of 20 kV with an error rate of 4%. The PANalytical- multipurpose Empyrean X-ray diffractometer was used to determine the bulk mineralogical composition of the retrieved minerals.