Commercially available primary human dermal fibroblasts (derived from adult skin of single donor) (NHDF-Ad, CC-2511; Lonza, Basel, Switzerland) were cultured as previously described [13 (link)] in complete fibroblast cell medium (Fibroblast Cell Basal Medium), supplemented with 2% fetal bovine serum, 0.1% r-human fibroblast growth factor-B, 0.1% insulin, 0.1% gentamicin sulphate/amphotericin-B (Clonetics™ FGM™-2 Bullet Kit™; Lonza, Basel, Switzerland). Sub-cultivation was performed at approximately 80% confluence using the “ReagentPack Subculture Reagent Kit” according to the manufacturer instructions (Lonza, Basel, Switzerland).
Reagentpack subculture reagent kit
Manufactured by Lonza
Sourced in Switzerland
ReagentPack Subculture Reagent Kit is a laboratory equipment product that provides a complete set of reagents for the subculture of cells. The kit includes essential components required for cell passaging and maintenance, such as buffers and growth media.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using reagentpack subculture reagent kit
1
Expansion of Primary Human Dermal Fibroblasts
2
Primary Adult Skin Fibroblast Cultivation
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Primary human dermal fibroblasts (adult skin) (Lonza, Basel, Switzerland) were seeded in 25 cm2 flasks and maintained in complete fibroblast cell medium (Fibroblast Cell Basal Medium), supplemented with 2% fetal bovine serum, 0.1% r-human fibroblast growth factor-B, 0.1% insulin, 0.1% gentamicin sulphate/amphotericin-B (“Bullet Kit”) (Lonza, Basel, Switzerland). Sub-cultivation was performed at approximately 80% confluence using the “ReagentPack Subculture Reagent Kit” (Lonza, Basel, Switzerland), according to the manufacturer’s instructions.
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