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Horseradish peroxidase hrp conjugated goat anti mouse igg sc 516102

Manufactured by Santa Cruz Biotechnology
Sourced in United States, China

Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-516102) is a secondary antibody conjugated with the enzyme horseradish peroxidase. This product is designed to detect and visualize mouse immunoglobulin G (IgG) in various immunoassays.

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2 protocols using horseradish peroxidase hrp conjugated goat anti mouse igg sc 516102

1

Quantifying Extracellular Matrix Proteins

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The antibodies against galectin-1(ab138513),and GAPDH(ab9485) were from Abcam (Cambridge, UK). The polyclonal antibodies against collagen I (bs-0578R), collagen III (bs-0549R), and fibronectin 1 (FN1)/Ugl-Y3 (bs-13455R) were from Bioss (Beijing, China). The goat anti-rabbit IgG (sc-2357) and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-516102) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The MTT assay kit and dimethyl sulfoxide were from Sigma-Aldrich (St. Louis, MO, USA). The Modified Masson’s Trichrome Stain Kit was from Beijing Solarbio Science & Technology Co. Ltd. (G1346; Beijing, China).
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2

Hedgehog Signaling Pathway Inhibitor ITD-1 Protocol

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ITD-1, a hedgehog signaling pathway inhibitor, was purchased from Macklin (1884570, Shanghai, China). The anti-GAL-1 antibody was purchased from Abcam (ab138513, Cambridge, UK), the anti-SMA-α antibody was purchased from Cell Signaling Technology (56856, Danvers, MA, USA), anti-E-cadherin (bs-10009R), anti-Vimentin (bs-0756R), anti-TGF-β1 (bs-0086R), and anti-p-Smad2/3(bs-8853R) antibodies were purchased from Bioss (Beijing, China), and the anti-glyceraldehyde phosphate dehydrogenase (GAPDH) antibody (sc-47724), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-516102), and goat anti-rabbit IgG (sc-2357) antibodies were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). The dimethyl sulfoxide (DMSO) and MTT assay kit and were provided by Sigma Biotechnology (St. Louis, MO, USA).
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