Mesoporphyrin 9

Manufactured by Frontier Scientific

Sourced in United States

Mesoporphyrin IX is a porphyrin compound. It is a chemical substance used in various scientific applications, particularly in the field of biochemistry and related areas of research.

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Lab products found in correlation

Formic acid, by Merck Group (2 mentions) Ferrous sulfate, by Merck Group (1 mentions) Dimethylformamide dmf, by Avantor (1 mentions) Protoporphyrin 9 cobalt chloride, by Merck Group (1 mentions) Magnesium sulfate, by Merck Group (1 mentions) Hemin from porcine, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Acetonitrile, by Carlo Erba (1 mentions)

Spelling variants of this product

N-Methyl-mesoporphyrin IX (NMM) (4 citations) N-methyl mesoporphyrin IX (4 citations) Mesoporphyrin IX dimethyl ester (3 citations) Mesoporphyrin IX dihydrochloride (2 citations) Sn (IV) mesoporphyrin IX dichloride (SnMP; (2 citations)

3 protocols using mesoporphyrin 9

Porphyrin Metabolic Pathway Analysis

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Formic acid (≥ 98%), tris(hydroxymethyl)aminomethane (Tris), ferrous sulfate, magnesium sulfate, and separate standards of protoporphyrin IX (purity ≥ 95%), protoporphyrin IX cobalt chloride, 5-aminolaevulinic acid hydrochloride (purity ≥ 97%), and hemin from porcine (purity ≥ 97%) were obtained from Sigma-Aldrich (Schnelldorf, Germany). HPLC-grade methanol, acetone, and acetonitrile were purchased from Rathburn Chemicals Ltd. (Walkerburn, Scotland). Analytical-grade hydrochloric acid (37%) and dimethylformamide (DMF) were obtained from VWR International (Fontenay-sous-Bois, France). Ethylenediaminetetraacetic acid disodium dihydrate salt (EDTA) and sodium chloride of reagent grade were acquired from Scherlab S.L. (Sentmenat, Spain). A Synergy 185 water purification system from Millipore (Molsheim, France) was used to produce deionized water at 18 MΩ cm. Porphyrin acid chromatographic marker kit (CMK-1A) containing 10 ± 1 nmol of each of six porphyrins (mesoporphyrin IX, coproporphyrin I, 5-carboxylporphyrin I, 6-carboxylporphyrin I, 7-carboxylporphyrin I, and uroporphyrin I) were obtained from Frontier Scientific Inc. (Logan, UT, USA). S. cerevisiae was used for method validation and it was obtained from Jästbolaget (Sollentuna, Sweden). E. coli NovaBlue was obtained from the Department of Biochemistry and Biophysics at Stockholm University.

Fyrestam J, & Östman C. (2017). Determination of heme in microorganisms using HPLC-MS/MS and cobalt(III) protoporphyrin IX inhibition of heme acquisition in Escherichia coli. Analytical and Bioanalytical Chemistry, 409(30), 6999-7010.

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Quantifying Heme and FECH Activity in PGRMC1

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The heme content of purified PGRMC1 was measured as its pyridine hemochromogen as previously described^{76 (link)}. FECH activity alone and in the presence of PGRMC1 was assayed with mesoporphyrin IX (Frontier Scientific, Logan, UT) and ferrous ammonium sulfate using the continuous direct spectroscopic method^{77 (link)}. Assays were performed in triplicate on at least two independent protein preparations.

Piel RB I.I.I., Shiferaw M.T., Vashisht A.A., Marcero J., Praissman J., Phillips J.D., Wohlschlegel J.A, & Medlock A.E. (2016). A Novel Role for Progesterone Receptor Membrane Component 1 (PGRMC1): A Partner and Regulator of Ferrochelatase. Biochemistry, 55(37), 5204-5217.

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Analytical Methods for Porphyrin Standards

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Analyses were performed using solvents of analytical grade: methanol (MeOH) RS HPLC Gold and Acetonitrile for HPLC gradient, ethyl acetate ACS for analysis and chloroform (CHCl3) RPE for analysis were purchased from Carlo Erba. Hydrochloric acid (HCl) 1 N and formic acid 98%‐100% for analyses were acquired from Merck (France or Darmstadt). Hydrochloric acid (HCl) 6 N and ammonium acetate for HPLC ≥ 99.0% were purchased from Sigma Aldrich. Acetic acid HiPerSolv Chromanorm for HPLC was acquired from VWR BDH Prolabo. Ultrapure water was obtained by Milli‐Q system from Millipore. Standards of coproporphyrin I HCl, coproporphyrin III HCl, protoporphyrine IX, mesoporphyrin IX were purchased from Frontier Scientific.

Josse G., Mias C., Le Digabel J., Filiol J., Ipinazar C., Villaret A., Gomiero C., Bevilacqua M., Redoules D., Nocera T., Saurat J, & Gontier E. (2020). High bacterial colonization and lipase activity in microcomedones. Experimental Dermatology, 29(2), 168-176.

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