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Ccl20 chemokine

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CCL20 chemokine is a protein that plays a role in the immune system. It is involved in the recruitment and activation of specific types of immune cells. The core function of CCL20 is to facilitate the migration and positioning of these immune cells within the body.

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Lab products found in correlation

Transwell plate, by Thermo Fisher Scientific (2 mentions) Casy tt cell counter, by Roche (1 mentions) Bovine serum albumin (bsa), by R&D Systems (1 mentions)

Close spelling variants of this product

CCL20

2 protocols using ccl20 chemokine

1

Chemotaxis Assay of BMDCs

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BMDCs from C57BL/6 or P2X7R-/- mice were added to the upper well of transwell plates (Fisher Scientific, Loughborough, UK) at 1 × 106 per well and 0.1% bovine serum albumin (BSA), CCL5 or CCL20 chemokine (both from R&D Systems) added to the bottom well in a two-fold serial dilution (1000 to 250 pg/ml). After incubating for 3 h at 37 °C, the number of cells in the bottom well were counted in triplicate using a CASY TT cell counter (Roche Innovatis AG, Germany). The percentage of migrated cells was calculated by subtracting background migration from the BSA only data.
Huang S.W., Walker C., Pennock J., Else K., Muller W., Daniels M.J., Pellegrini C., Brough D., Lopez-Castejon G, & Cruickshank S.M. (2016). P2X7 receptor-dependent tuning of gut epithelial responses to infection. Immunology and Cell Biology, 95(2), 178-188.
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2

Chemotaxis Assay for Bone Marrow-Derived Cells

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BMDCs from C57BL/6 or P2X7R−/− mice were added to the upper well of transwell plates (Fisher Scientific, Loughborough, UK) at 1 × 106 per well and 0.1% bovine serum albumin, CCL5 or CCL20 chemokine (both from R&D Systems) added to the bottom well in a twofold serial dilution (1000–250 pg ml−1). After incubating for 3 h at 37 °C, the number of cells in the bottom well were counted in triplicate using a CASY TT Cell Counter (Roche Innovatis AG, Bielefeld, Germany). The percentage of migrated cells was calculated by subtracting background migration from the bovine serum albumin only data.
Huang S.W., Walker C., Pennock J., Else K., Muller W., Daniels M.J., Pellegrini C., Brough D., Lopez-Castejon G, & Cruickshank S.M. (2016). P2X7 receptor-dependent tuning of gut epithelial responses to infection. Immunology and Cell Biology, 95(2), 178-188.
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