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7d dslr camera

Manufactured by Canon
Sourced in Japan

The Canon 7D is a DSLR camera with a 18.0 megapixel APS-C CMOS sensor, 19-point autofocus system, and 8 frames per second continuous shooting. It is capable of recording 1080p Full HD video at 30 frames per second.

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Lab products found in correlation

2 protocols using 7d dslr camera

1

Cloning and Characterizing Zebrafish sorcs3 and ccdc147

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We cloned fragments of sorcs3 and ccdc147 from zebrafish cDNAs by RT-PCR and subcloned them into pGEMT-easy vectors for antisense probe synthesis. Primer pairs used are as follows: sorcs3 (forward: GTC​GCC​AAT​GCA​AGT​GAA​TTA​CGC; reverse: TTT​CCA​GAC​CAG​TAC​ACG​ACT​GCG​T) and ccdc147 (forward: GAC​GAC​AGT​ACG​TTG​GAA​ACC​ATG​G; reverse: CGG​TGG​CTT​TAG​TAA​GGT​TTT​CCC​G). Whole-mount in-situ hybridization (WISH) was performed as described using digoxigenin (DIG)-labeled antisense RNA probe (Thisse and Thisse, 2008 (link)). Stained embryos were mounted in glycerol, observed under a Leica S8AP0 stereomicroscope (Leica Microsystems, Wetzlar, Germany), and photographed using a Canon 7D DSLR camera (Canon, Tokyo, Japan).
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2

Zebrafish sorcs3 and ccdc147 expression

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DNA fragments of sorcs3 and ccdc147 was cloned from zebrafish cDNAs by RT-PCR and subcloned into pGEMT-easy vectors for antisense probe synthesis. Primer pairs used are as following: sorcs3 (forward: GTCGCCAATGCAAGTGAATTACGC; reverse: TTTCCAGACCAGTACACGACTGCGT) and ccdc147 (forward: GACGACAGTACGTTGGAAACCATGG; reverse: CGGTGGCTTTAGTAAGGTTTTCCCG). Whole-mount in situ hybridization (WISH) was performed as described using digoxigenin (DIG)-labeled antisense RNA probe [58] .
Stained embryos were mounted in glycerol, observed under a Leica S8AP0 stereomicroscope (Leica Microsystems, Wetzlar, Germany) and photographed using a Canon 7D DSLR camera (Canon, Tokyo, Japan).
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