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Seqcap ez exome library v2.0 kit

Manufactured by Roche
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The SeqCap EZ Exome Library v2.0 kit is a DNA library preparation kit designed for targeted enrichment of coding exons. It enables the capture and sequencing of the human exome, which represents the protein-coding regions of the genome.

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Lab products found in correlation

Sureselect target enrichment system, by Agilent Technologies (2 mentions) Hiseq 2000, by Illumina (2 mentions)

Close spelling variants of this product

SeqCap EZ Library (19 citations) SeqCap EZ Exome v2.0 (5 citations) SeqCap EZ Human Exome Library v2.0 kit (2 citations)

2 protocols using seqcap ez exome library v2.0 kit

1

Whole Exome Sequencing Library Preparation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For GLD1, sequencing libraries were made following the protocol from Roche/Nimblegen’s SeqCap EZ Exome Library v2.0 kit (Roche NimbleGen, Inc). The libraries were then sequenced on a HiSeq2000 (Illumina), with paired-end, 101bp reads. For GLD2 and GLD3, whole exome capture was performed using the SureSelect Target Enrichment System (Agilent). This was followed by sequencing on a HiSeq2000 (Illumina) with 100bp paired end reads. Sequence reads for all families were aligned to the reference genome (hg19) using Novoalign (Novocraft Technologies SdnBhd). Duplicate reads, resulting from PCR clonality or optical duplicates, and reads mapping to multiple locations were excluded from downstream analysis. Depth and breadth of sequence coverage were calculated with custom scripts and the BedTools package26 (link).
Fotiou E., Martin-Almedina S., Simpson M.A., Lin S., Gordon K., Brice G., Atton G., Jeffery I., Rees D.C., Mignot C., Vogt J., Homfray T., Snyder M.P., Rockson S.G., Jeffery S., Mortimer P.S., Mansour S, & Ostergaard P. (2015). Novel mutations in PIEZO1 cause an autosomal recessive generalized lymphatic dysplasia with non-immune hydrops fetalis. Nature Communications, 6, 8085.
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2

Whole Exome Sequencing Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For GLD1, sequencing libraries were made following the protocol from Roche/Nimblegen's SeqCap EZ Exome Library v2.0 kit (Roche NimbleGen, Inc). The libraries were then sequenced on a HiSeq2000 (Illumina), with paired end, 101-bp reads. For GLD2 and GLD3, whole exome capture was performed using the SureSelect Target Enrichment System (Agilent). This was followed by sequencing on a HiSeq2000 (Illumina) with 100-bp paired end reads. Sequence reads for all families were aligned to the reference genome (hg19) using Novoalign (Novocraft Technologies SdnBhd). Duplicate reads, resulting from PCR clonality or optical duplicates and reads mapping to multiple locations were excluded from downstream analysis. Depth and breadth of sequence coverage were calculated with custom scripts and the BedTools package26 (link).
Fotiou E., Martin-Almedina S., Simpson M.A., Lin S., Gordon K., Brice G., Atton G., Jeffery I., Rees D.C., Mignot C., Vogt J., Homfray T., Snyder M.P., Rockson S.G., Jeffery S., Mortimer P.S., Mansour S, & Ostergaard P. (2015). Novel mutations in PIEZO1 cause an autosomal recessive generalized lymphatic dysplasia with non-immune hydrops fetalis. Nature Communications, 6, 8085.
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