Log In Sign up
The largest database of trusted experimental protocols

Block it u6 adenoviral rnai system

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Block-it U6 adenoviral RNAi system is a laboratory equipment designed for the delivery of small interfering RNA (siRNA) into target cells. It facilitates the knockdown of gene expression through RNA interference (RNAi) using an adenoviral vector.

Automatically generated - may contain errors

Lab products found in correlation

293a cells, by Thermo Fisher Scientific (2 mentions) Mission shrna library, by Merck Group (2 mentions)

Close spelling variants of this product

BLOCK-iT

2 protocols using block it u6 adenoviral rnai system

1

Adenoviral Knockdown of Frizzled Receptors

Check if the same lab product or an alternative is used in the 5 most similar protocols
All shRNAs were purchased from Sigma (MISSION shRNA library). The KD efficiency was validated as described in Extended Data Fig. 8c, d. ShRNA sequences showing the highest efficiency were selected to generate adenoviruses. Adenoviruses expressing a control shRNA (5’-CTGGACTTCCAGAAGAACA-3’), shRNAs against mouse FZD1 (shRNA#2: 5’-TGGTGTGCAACGACAAGTTTG-3’), or FZD2 (shRNA#5: 5’-CGCTTCTCAGAGGACGGTTAT-3’) were constructed using the Block-it U6 adenoviral RNAi system (Life Technologies), followed by viral packaging and multiple rounds of amplification in 293A cells (Life Technologies).
Tao L., Zhang J., Meraner P., Tovaglieri A., Wu X., Gerhard R., Zhang X., Stallcup W.B., Miao J., He X., Hurdle J.G., Breault D.T., Brass A.L, & Dong M. (2016). Frizzled are colonic epithelial receptors for Clostridium difficile toxin B. Nature, 538(7625), 350-355.
+ Open protocol
+ Expand
2

Adenoviral Knockdown of Frizzled Receptors

Check if the same lab product or an alternative is used in the 5 most similar protocols
All shRNAs were purchased from Sigma (MISSION shRNA library). The KD efficiency was validated as described in Extended Data Fig. 8c, d. ShRNA sequences showing the highest efficiency were selected to generate adenoviruses. Adenoviruses expressing a control shRNA (5’-CTGGACTTCCAGAAGAACA-3’), shRNAs against mouse FZD1 (shRNA#2: 5’-TGGTGTGCAACGACAAGTTTG-3’), or FZD2 (shRNA#5: 5’-CGCTTCTCAGAGGACGGTTAT-3’) were constructed using the Block-it U6 adenoviral RNAi system (Life Technologies), followed by viral packaging and multiple rounds of amplification in 293A cells (Life Technologies).
Tao L., Zhang J., Meraner P., Tovaglieri A., Wu X., Gerhard R., Zhang X., Stallcup W.B., Miao J., He X., Hurdle J.G., Breault D.T., Brass A.L, & Dong M. (2016). Frizzled are colonic epithelial receptors for Clostridium difficile toxin B. Nature, 538(7625), 350-355.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!