D camp

Manufactured by Bio-Techne

Sourced in United Kingdom

D-cAMP is a cyclic adenosine monophosphate (cAMP) analog produced by Bio-Techne. It serves as a biological research tool for the study of cAMP-mediated signaling pathways.

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Lab products found in correlation

Anti gadph, by Merck Group (1 mentions) Anti pka, by Cell Signaling Technology (1 mentions) Anti cbs, by Santa Cruz Biotechnology (1 mentions) 8 br cgmp, by Bio-Techne (1 mentions) Anti pkg, by Cell Signaling Technology (1 mentions) Anti ha, by Santa Cruz Biotechnology (1 mentions) Adenosine, by Merck Group (1 mentions) Forskolin, by Enzo Life Sciences (1 mentions) Human serum, by Merck Group (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Amp cp, by Merck Group (1 mentions) Cay10598, by Cayman Chemical (1 mentions) Butaprost, by Cayman Chemical (1 mentions) Il 10, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)

2 protocols using d camp

Cyclic Nucleotide Regulation in T24 Cells

Cited 1 time

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T24 cells were starved for 16 hours, incubated with 100 μM of 8-Br-cGMP or d-cAMP (Tocris, UK) for 5, 15 or 30 min and then used for immunoprecipitation assay. Samples were analyzed as previously described [28 (link),29 (link)]. The eluted proteins were used in cell free kinase assays, phosphorylation assay in T24 cells and western blotting analysis.
Aliquots of immunocomplexes and protein samples (30μg) were resolved by 12% SDS-PAGE and analyzed as previously described [30 (link),31 (link)]. The membranes then were challenged with anti-CBS (Santa Cruz Biotechnology), anti-HA (Santa Cruz Biotechnology), anti-PKA (Cell Signaling Technology), anti-PKG (Cell Signaling Technology), anti-CSE (Novus Biologicals), anti-GADPH (Sigma Aldrich) and anti-phospho-(Ser/Thr) (anti-pS/T; Cell Signaling Technology). The proteins were visualized with enhanced chemiluminescence detection reagent according to the manufacturer's instructions (Pierce, Rockford, IL, USA).

d’Emmanuele di Villa Bianca R., Mitidieri E., Esposito D., Donnarumm E., Russo A., Fusco F., Ianaro A., Mirone V., Cirino G., Russo G, & Sorrentino R. (2015). Human Cystathionine-β-Synthase Phosphorylation on Serine227 Modulates Hydrogen Sulfide Production in Human Urothelium. PLoS ONE, 10(9), e0136859.

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Immunomodulatory Cytokine and Receptor Agonist Assay

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Human and murine GM-CSF, IL-6, IL-4, and IL-10 were obtained from Peprotech. PGE₂ and D-cAMP were obtained from Tocris. The EP2 receptor agonist Butaprost and EP4 receptor agonist CAY10598 were obtained from Cayman. Forskolin was obtained from Enzo Life Sciences. STAT3 inhibitor S3I-201 and CREB inhibitor 666-15 were obtained from Calbiochem. Adenosine, ADA, AMP, AMP-CP, erythro-9-(2-hydroxy-3-nonyl) adenine (ADA inhibitor), OVA, and human serum were obtained from Sigma-Aldrich. Neutralizing anti–IL-10 Ab was obtained from R&D Systems. Carboxyfluorescein succinimidyl ester (CFSE) was obtained from Thermo Fisher Scientific.

Sarkar O.S., Donninger H., Al Rayyan N., Chew L.C., Stamp B., Zhang X., Whitt A., Li C., Hall M., Mitchell R.A., Zippelius A., Eaton J., Chesney J.A, & Yaddanapudi K. (2023). Monocytic MDSCs exhibit superior immune suppression via adenosine and depletion of adenosine improves efficacy of immunotherapy. Science Advances, 9(26), eadg3736.

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