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Rat ifn γ elisa kit

Manufactured by MultiSciences Biotech
Sourced in China

The Rat IFN-γ ELISA kit is a laboratory tool used to detect and quantify the levels of interferon-gamma (IFN-γ) in rat biological samples. It is an enzyme-linked immunosorbent assay (ELISA) that employs antibody-based techniques to measure the concentration of this cytokine.

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2 protocols using rat ifn γ elisa kit

1

Quantifying Inflammatory Cytokines in Rat Serum

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The blood samples were harvested from the carotid artery, and the heparins were added as an anticoagulant. Subsequently, blood samples were centrifuged at 3000 rpm for 15 min (4°C), and the supernatants were collected. The serum concentrations of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interferon-γ (IFN-γ) were measured via the rat TNF-α ELISA kit (Thermo Fisher Scientific, MA, USA), rat IL-1β ELISA kit (Multi Sciences Biotech, Hangzhou, China), and rat IFN-γ ELISA kit (Multi Sciences Biotech, Hangzhou, China) according to the manufacturer's protocols.
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2

Cytokine Profiling in Cell Cultures

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TNF-α, IL-1β, IL-10, IL-4, G-CSF and TGF-β1 levels in culture supernatants were measured using a Mouse TNF-α ELISA Kit (MULTI SCIENCES, China), Mouse IL-1β ELISA Kit (MULTI SCIENCES, China), Mouse IL-10 ELISA Kit (Angle Gene, China), Mouse IL-4 ELISA Kit (Angle Gene, China), Mouse G-CSF/CSF3 ELISA Kit (Sigma-Aldrich, USA), and mouse TGF-β1 ELISA kit (KeyGEN BioTech Co., Ltd, China). Moreover, IFN-γ levels in intestinal tissues were measured using a rat IFN-γ ELISA kit (MULTI SCIENCES, China).
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