Proteins were separated by 10 % (w/v) NuPAGE® Gel (Invitrogen, Carlsbad, CA, USA) and blotted onto a PVDF membrane (GE Healthcare, Little Chalfont, UK) using the Xcell II™ Blot Module (Invitrogen, Carlsbad, CA, USA). The FBP/SBPase protein was detected using a mouse monoclonal FBP/SBPase antibody [24 (link)] and anti-mouse IgG–horseradish peroxidase (HRP) conjugate (Bio-Rad, Hercules, CA, USA) as the secondary antibody. Protein bands were detected using the enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK).
Anti mouse igg horseradish peroxidase hrp conjugate
Manufactured by Bio-Rad
Sourced in United States, France
The Anti-mouse IgG–horseradish peroxidase (HRP) conjugate is a laboratory reagent used for the detection and quantification of mouse immunoglobulin G (IgG) in various analytical techniques. The conjugate consists of an anti-mouse IgG antibody covalently linked to the enzyme horseradish peroxidase, which serves as a reporter molecule to generate a colorimetric or chemiluminescent signal when exposed to the appropriate substrate.
Automatically generated - may contain errors
Lab products found in correlation
Nupage gel, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by GE Healthcare (1 mentions)
Xcell 2 blot module, by Thermo Fisher Scientific (1 mentions)
Enhanced chemiluminescence detection system, by GE Healthcare (1 mentions)
Chemidoc xrs imaging system, by Bio-Rad (1 mentions)
Image lab software, by Bio-Rad (1 mentions)
Luminata classico western hrp substrate, by Merck Group (1 mentions)
T5168, by Merck Group (1 mentions)
Sc 8005, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti mouse igg horseradish peroxidase hrp conjugate
1
Western Blot Analysis of FBP/SBPase
2
Immunoblotting Analysis of Liver Proteins
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Proteins prepared from liver were separated on SDS-10% polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane. Immunoblotting was performed as previously described (Naville et al., 2013) . Primary antibodies included mouse monoclonal antibodies directed against Esr1 (dilution 1:1000; sc-8005; Santa-Cruz Biotechnology, Cliniscience, Nanterre, France) or α-tubulin (dilution 1:2000; T5168; Sigma-Aldrich). The secondary antibodies were anti-mouse IgG horseradish peroxidase (HRP) conjugate (Bio-Rad, Marnes-la-coquette, France). Blots were revealed using the Luminata Classico Western HRP substrate (Millipore, Molsheim, France) and detection was made using the ChemiDocXRS+imaging system (BioRad). Results were analyzed with Image Lab Software (BioRad). Data were normalized relatively to α -tubulin.
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