RNA was prepared using RNeasy Micro Kit (Qiagen). RNA quantity and quality was assessed using a Nanodrop 1000 Spectrometer (Peqlab, Erlangen, Germany) and Agilent 2100 Bioanalyzer. From in vitro-generated cells (M1-, M2-macrophages) and CD14+ monocytes, three replica pools (each consisting of RNA from 5 different HD) were generated. RNA from flow-sorted cells was not pooled (Table S2 ). RNA (30 ng of each replica pool; 0.5–15 ng of sorted cells) was subsequently amplified and converted into cDNA by a linear amplification method using WT-Ovation PicoSL System in combination with the Encore® Biotin Module (both from NuGen, San Carlos, CA, USA). cDNA was hybridized to Affymetrix GeneChip® Human Gene 1.0 ST Arrays.
Wt ovation picosl system
Manufactured by Tecan
Sourced in United States
The WT-Ovation PicoSL System is a laboratory instrument designed for amplifying and preparing RNA samples for downstream analysis. The system utilizes a proprietary technology to generate amplified cDNA from small amounts of input RNA, enabling researchers to study gene expression patterns from limited samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using wt ovation picosl system
1
RNA Extraction and Amplification for Microarray
2
Transcriptome Analysis of Macrophage Subtypes
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RNA was prepared using RNeasy micro kit (Qiagen). RNA quantity and quality was assessed using a Nanodrop 1000 Spectrometer (Peqlab) and Agilent 2100
Bioanalyzer. From in vitro-generated cells (M1-, M2-macrophages) and CD14 + monocytes, three replica pools (each consisting of RNA from 5 different HD) were generated. RNA from flow-sorted cells was not pooled (table S2). RNA (30 ng of each replica pool; 0.5-15 ng of sorted cells) was subsequently amplified and converted into cDNA by a linear amplification method using WT-Ovation PicoSL System in combination with the Encore® Biotin Module (both from NuGen, San Carlos, US).
cDNA was hybridized to Affymetrix GeneChip® Human Gene 1.0 ST Arrays.
Bioanalyzer. From in vitro-generated cells (M1-, M2-macrophages) and CD14 + monocytes, three replica pools (each consisting of RNA from 5 different HD) were generated. RNA from flow-sorted cells was not pooled (table S2). RNA (30 ng of each replica pool; 0.5-15 ng of sorted cells) was subsequently amplified and converted into cDNA by a linear amplification method using WT-Ovation PicoSL System in combination with the Encore® Biotin Module (both from NuGen, San Carlos, US).
cDNA was hybridized to Affymetrix GeneChip® Human Gene 1.0 ST Arrays.
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