Mouse anti erk1 2

Manufactured by Merck Group

Sourced in United States

The Mouse anti-ERK1/2 is a laboratory reagent that recognizes the extracellular signal-regulated kinase 1 and 2 (ERK1/2) proteins. ERK1/2 are key components of the mitogen-activated protein kinase (MAPK) signaling pathway, which plays a crucial role in cellular processes such as proliferation, differentiation, and survival. This antibody can be used to detect and study the expression and activation of ERK1/2 in various experimental systems.

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Close spelling variants of this product

ERK1/2 (49 citations) Anti-ERK1/2 (24 citations) Mouse anti-phospho-ERK1/2 antibody (2 citations)

3 protocols using mouse anti erk1 2

Comprehensive Antibody Panel for Cellular Analysis

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The following primary antibodies were employed for either immunofluorescence or western blotting: mouse anti-ARH-I (for IF 1:250, for WB 1:1000; cod. ab45768; Abcam, Cambridge, UK), mouse anti-p21 (for IF 1:100, for WB 1:200; cod. sc-817; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-LC3 (1:2000; cod. L7543; Sigma-Aldrich), rabbit anti-p62 (1:500; cod. 8025; Cell Signaling, Danvers, MA, USA), mouse anti-β-actin (1:2000; cod. A5441; Sigma-Aldrich), mouse anti-BECLIN-1 (1:500; cod. 612112; BD Biosciences, Franklin Lakes, NJ, USA), goat anti-BECLIN-1 (1:250; cod. sc-10086; Santa Cruz Biotechnology), rabbit anti-VPS34 (1:500; cod. 4263; Cell signaling), mouse anti-STAT3 (1:500; cod. 9139; Cell Signaling), rabbit anti-phospho (Tyr705) STAT3 (1:1000; cod. 9145; Cell Signaling), rabbit anti-Ki-67 (1:100; cod. HPA001164; Sigma-Aldrich), mouse anti-BCL-2 (1:500; cod. 15071; Cell Signaling), mouse anti-IL-6R (1:1000; cod. AHR0061; Invitrogen, Waltham, MA, USA), rabbit anti-cyclin D1 (1:500; cod. 2978; Cell Signaling), rabbit anti-p38 (1:500; cod. 9212; Cell Signaling), rabbit anti-GAPDH (1:1000, cod. G9545, Sigma Aldrich), and mouse anti-ERK1/2 (1:500; cod. 05-1152; Millipore, Burlington, MA, USA).

Esposito A., Ferraresi A., Salwa A., Vidoni C., Dhanasekaran D.N, & Isidoro C. (2022). Resveratrol Contrasts IL-6 Pro-Growth Effects and Promotes Autophagy-Mediated Cancer Cell Dormancy in 3D Ovarian Cancer: Role of miR-1305 and of Its Target ARH-I. Cancers, 14(9), 2142.

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Western Blotting and Immunofluorescence Protocols

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The following primary antibodies were employed for western blotting: mouse anti-β-tubulin (1:1000, cod. T5201; Sigma-Aldrich Corp.), mouse anti-β-actin (1:2000, cod. A5441; Sigma-Aldrich Corp.), rabbit anti-GAPDH (1:1000, cod. G9545; Sigma-Aldrich Corp.), mouse anti-cathepsin D (1:100, cod. IM03; Calbiochem, St. Louis, MO, USA), rabbit anti phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) (1:500, cod. 05-797R; Millipore, Burlington, MA, USA) and mouse anti-ERK1/2 (1:500, cod. 05-1152; Millipore). Secondary antibodies employed for immunoblotting were purchased as follows: Horse Radish Peroxidase-conjugated goat anti-mouse IgG (1:10,000, cod. 170–6516; Bio-Rad, Hercules, CA, USA) and Horse Radish Peroxidase-conjugated goat anti-rabbit IgG (1: 10,000, cod. 170–6515: Bio-Rad, Hercules, CA, USA). The following primary antibodies were employed for immunofluorescence: mouse anti-p21 (1:100, cod. sc-817; Santa Cruz Biotechnology, Dallas, TX, USA) and rabbit anti-Ki-67 (1:100, cod. HPA001164; Sigma-Aldrich). Secondary antibodies used for immunofluorescence were purchased as follows: goat-Anti Rabbit IgG Alexa FluorTM Plus 488 (1:1000, cod. A32731; Invitrogen) and Goat-Anti Mouse IgG Alexa FluorTM Plus 555 (1:1000, cod. A32727; Invitrogen).

Secomandi E., Salwa A., Vidoni C., Ferraresi A., Follo C, & Isidoro C. (2022). High Expression of the Lysosomal Protease Cathepsin D Confers Better Prognosis in Neuroblastoma Patients by Contrasting EGF-Induced Neuroblastoma Cell Growth. International Journal of Molecular Sciences, 23(9), 4782.

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Antibody Panel for Cell Signaling Analysis

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The following primary antibodies (at the dilution indicated) were used for either immunofluorescence or Western blotting: mouse anti-p21 (1:100, cod. B1313; Santa Cruz, Biotechnology, Dallas, TX, USA); rabbit anti-Ki67 (1:100, cod. HPA001164; Sigma-Aldrich, St. Louis, MO, USA); rabbit anti-phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187) (1:500, cod. 05–797R; Millipore, Burlington, MA, USA); mouse anti-ERK1/2 (1:500, cod. 05–1152; Millipore, Burlington, MA, USA); rabbit anti-phospho-S6 (Ser235/236) (1:500, cod. 4856; Cell Signaling, Danvers, MA, USA); rabbit anti-S6 (1:500, cod. 2217; Cell Signaling, Danvers, MA, USA); mouse anti-N-Cadherin (1:50, cod. 610920; BD Biosciences, Franklin Lakes, NJ); mouse anti-E-Cadherin (1:50, cod. 610404; BD Biosciences, Franklin Lakes, NJ); rabbit anti-LC3 (1:1000, cod. L7543; Sigma-Aldrich, St. Louis, MO, USA); mouse anti-LAMP1 (1:1000, cod. 555798; BD, Biosciences, Franklin Lakes, NJ); rabbit anti- β-Catenin (1:500, cod. PA5-77934; Invitrogen, Paisley, UK); mouse anti-β-Actin (1:2000, cod. A5441; Sigma-Aldrich, St. Louis, MO, USA); mouse anti-β-Tubulin (1:1000, cod. T5326; Sigma-Aldrich, St. Louis, MO, USA); rabbit anti-GAPDH (1:1000, cod. G9545; Sigma-Aldrich, St. Louis, MO, USA).

Vallino L., Garavaglia B., Visciglia A., Amoruso A., Pane M., Ferraresi A, & Isidoro C. (2023). Cell-free Lactiplantibacillus plantarum OC01 supernatant suppresses IL-6-induced proliferation and invasion of human colorectal cancer cells: Effect on β-Catenin degradation and induction of autophagy. Journal of Traditional and Complementary Medicine, 13(2), 193-206.

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