Preparation of protein extracts and Western blot analysis were performed as described previously19 (link). Anti-GFP antibody from mouse IgG1κ (clones 7.1 and 13.1) (Roche) was used. Detection and quantification were carried out by using the Odyssey Imaging Systems (LI-COR Biosciences). Statistical analysis was performed with Excel (Microsoft).
Anti gfp antibody from mouse igg1κ clones 7.1 and 13 1
Manufactured by Roche
The Anti-GFP antibody from mouse IgG1κ (clones 7.1 and 13.1) is a laboratory reagent used for the detection and purification of green fluorescent protein (GFP) and related fluorescent proteins. The antibody specifically binds to GFP, allowing for its identification and isolation in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Odyssey imaging system, by LI COR (2 mentions)
Immobilon western, by Merck Group (1 mentions)
Anti myc monoclonal antibody 9e10, by Santa Cruz Biotechnology (1 mentions)
Anti gfp monoclonal antibody jl 8, by Takara Bio (1 mentions)
Anti phospho ampkα monoclonal antibody 40h9, by Cell Signaling Technology (1 mentions)
Anti snf1 polyclonal antibody yk 16, by Santa Cruz Biotechnology (1 mentions)
Anti mcm2 polyclonal antibody n 19, by Santa Cruz Biotechnology (1 mentions)
Las 4000, by Fujifilm (1 mentions)
2 protocols using anti gfp antibody from mouse igg1κ clones 7.1 and 13 1
1
Western Blot Analysis of GFP Expression
2
Western Blot Analysis of GFP-tagged Proteins
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Preparation of protein extracts and Western blot analysis were performed as described previously [10 (link)]. WIDE RANGE Gel Preparation Buffer(4x) for PAGE (Nakalai) was used to detect degradation of GFP-tagged proteins. Anti-GFP monoclonal antibody JL-8 (Clontech), anti-GFP antibody from mouse IgG1κ (clones 7.1 and 13.1) (Roche), anti-phospho-AMPKα monoclonal antibody 40H9 (Cell Signaling), anti-Snf1 polyclonal antibody yk-16 (Santa Cruz), anti-Myc monoclonal antibody 9E10 (Santa Cruz) and anti-Mcm2 polyclonal antibody N-19 (Santa Cruz) were used. Detection was carried out by using a LAS-4000 (Fuji Film) with Immobilon Western (Merck Millipore) or the Odyssey Imaging Systems (LI-COR Biosciences). Signal intensities were quantified by the Odyssey Imaging Systems, and statistical analysis was performed with Excel (Microsoft).
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